論文 - 詳細
| RRC ID | 76286 |
|---|---|
| 著者 | Sang L, Dong R, Liu R, Hao Q, Bai W, Sun J. |
| タイトル | Caenorhabditis elegans NHR-14/HNF4α regulates DNA damage-induced apoptosis through cooperating with cep-1/p53. |
| ジャーナル | Cell Commun Signal |
| Abstract |
BACKGROUND:Nuclear hormone receptors are involved in transcriptional regulation and many important cellular processes including development and metabolism. However, its role in DNA damage-induced apoptosis remains elusive. METHODS:Synchronized young adult animals were irradiated with different doses of gamma-Ray, and then put back to culture at 20 °C. Germline cell apoptosis was scored at different time point. RESULTS:Deletion of nhr-14 led to decreased DNA damage-induced germline apoptosis, but not the physiological programmed cell death. We also demonstrate that nhr-14 functions downstream of the DNA damage checkpoint pathway. Moreover, we show that nhr-14 regulates egl-1 and ced-13 transcription upon DNA damage. Mechanistically, NHR-14 forms a complex with CEP-1/p53 and binds directly to the egl-1 promoter to promote egl-1 transcription.. CONCLUSIONS:Our results indicate that NHR-14/HNF4α cooperates with CEP-1/p53 to regulate DNA damage-induced apoptosis. Video abstract. |
| 巻・号 | 20(1) |
| ページ | 135 |
| 公開日 | 2022-9-1 |
| DOI | 10.1186/s12964-022-00920-5 |
| PII | 10.1186/s12964-022-00920-5 |
| PMID | 36050770 |
| PMC | PMC9438139 |
| MeSH | Animals Apoptosis Caenorhabditis elegans* / genetics Caenorhabditis elegans* / metabolism Caenorhabditis elegans Proteins* / genetics DNA Damage Tumor Suppressor Protein p53 / metabolism |
| リソース情報 | |
| 線虫 | tm1473 tm1145 tm1125 |