論文 - 詳細
| RRC ID | 76495 |
|---|---|
| 著者 | Chestnut B, Casie Chetty S, Koenig AL, Sumanas S. |
| タイトル | Single-cell transcriptomic analysis identifies the conversion of zebrafish Etv2-deficient vascular progenitors into skeletal muscle. |
| ジャーナル | Nat Commun |
| Abstract |
Cell fate decisions involved in vascular and hematopoietic embryonic development are still poorly understood. An ETS transcription factor Etv2 functions as an evolutionarily conserved master regulator of vasculogenesis. Here we report a single-cell transcriptomic analysis of hematovascular development in wild-type and etv2 mutant zebrafish embryos. Distinct transcriptional signatures of different types of hematopoietic and vascular progenitors are identified using an etv2ci32Gt gene trap line, in which the Gal4 transcriptional activator is integrated into the etv2 gene locus. We observe a cell population with a skeletal muscle signature in etv2-deficient embryos. We demonstrate that multiple etv2ci32Gt; UAS:GFP cells differentiate as skeletal muscle cells instead of contributing to vasculature in etv2-deficient embryos. Wnt and FGF signaling promote the differentiation of these putative multipotent etv2 progenitor cells into skeletal muscle cells. We conclude that etv2 actively represses muscle differentiation in vascular progenitors, thus restricting these cells to a vascular endothelial fate. |
| 巻・号 | 11(1) |
| ページ | 2796 |
| 公開日 | 2020-6-3 |
| DOI | 10.1038/s41467-020-16515-y |
| PII | 10.1038/s41467-020-16515-y |
| PMID | 32493965 |
| PMC | PMC7271194 |
| MeSH | Animals Animals, Genetically Modified Biomarkers / metabolism Blood Vessels / cytology* Cell Differentiation / genetics Cell Movement Embryo, Nonmammalian / metabolism Fibroblast Growth Factors / metabolism Gene Expression Profiling* Green Fluorescent Proteins / metabolism Heat-Shock Response Models, Biological Muscle, Skeletal / cytology* Mutation / genetics Single-Cell Analysis* Somites / metabolism Stem Cells / metabolism* Transcription, Genetic Wnt Signaling Pathway Zebrafish / embryology Zebrafish / genetics* Zebrafish Proteins / deficiency* Zebrafish Proteins / genetics Zebrafish Proteins / metabolism |
| IF | 12.121 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 11 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | UAS:GFP |