論文 - 詳細
| RRC ID | 77025 |
|---|---|
| 著者 | Zeng J, Tang SY, Toh LL, Wang S. |
| タイトル | Generation of "Off-the-Shelf" Natural Killer Cells from Peripheral Blood Cell-Derived Induced Pluripotent Stem Cells. |
| ジャーナル | Stem Cell Reports |
| Abstract |
Current donor cell-dependent strategies can only produce limited "made-to-order" therapeutic natural killer (NK) cells for limited patients. To provide unlimited "off-the-shelf" NK cells that serve many recipients, we designed and demonstrated a holistic manufacturing scheme to mass-produce NK cells from induced pluripotent stem cells (iPSCs). Starting with a highly accessible human cell source, peripheral blood cells (PBCs), we derived a good manufacturing practice-compatible iPSC source, PBC-derived iPSCs (PBC-iPSCs) for this purpose. Through our original protocol that excludes CD34+ cell enrichment and spin embryoid body formation, high-purity functional and expandable NK cells were generated from PBC-iPSCs. Above all, most of these NK cells expressed no killer cell immunoglobulin-like receptors (KIRs), which renders them unrestricted by recipients' human leukocyte antigen genotypes. Hence, we have established a practical "from blood cell to stem cells and back with less (less KIRs)" strategy to generate abundant "universal" NK cells from PBC-iPSCs for a wide range of patients. |
| 巻・号 | 9(6) |
| ページ | 1796-1812 |
| 公開日 | 2017-12-12 |
| DOI | 10.1016/j.stemcr.2017.10.020 |
| PII | S2213-6711(17)30475-7 |
| PMID | 29173894 |
| PMC | PMC5785702 |
| MeSH | Cell Culture Techniques / methods* Cell Differentiation / genetics* Cell Proliferation / genetics Embryoid Bodies / cytology Gene Expression Regulation, Developmental / genetics Humans Induced Pluripotent Stem Cells / cytology* Induced Pluripotent Stem Cells / metabolism Killer Cells, Natural / cytology* Killer Cells, Natural / metabolism Peripheral Blood Stem Cells / cytology Peripheral Blood Stem Cells / metabolism Receptors, KIR / genetics |
| IF | 6.032 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 50 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| ヒト・動物細胞 | OP9/DLL1 |