| RRC ID |
77267
|
| 著者 |
Baba K, Uemura K, Nakazato R, Ijaz F, Takahashi S, Ikegami K.
|
| タイトル |
Δ3-tubulin impairs mitotic spindle morphology and increases nuclear size in pancreatic cancer cells.
|
| ジャーナル |
Med Mol Morphol
|
| Abstract |
Cancer cell proliferation is affected by post-translational modifications of tubulin. Especially, overexpression or depletion of enzymes for modifications on the tubulin C-terminal region perturbs dynamic instability of the spindle body. Those modifications include processing of C-terminal amino acids of α-tubulin; detyrosination, and a removal of penultimate glutamic acid (Δ2). We previously found a further removal of the third last glutamic acid, which generates so-called Δ3-tubulin. The effects of Δ3-tubulin on spindle integrities and cell proliferation remain to be elucidated. In this study, we investigated the impacts of forced expression of Δ3-tubulin on the structure of spindle bodies and cell division in a pancreatic cancer cell line, PANC-1. Overexpression of HA-tagged Δ3-tubulin impaired the morphology and orientation of spindle bodies during cell division in PANC-1 cells. In particular, spindle bending was most significantly increased. Expression of EGFP-tagged Δ3-tubulin driven by the endogenous promoter of human TUBA1B also deformed and misoriented spindle bodies. Spindle bending and condensation defects were significantly observed by EGFP-Δ3-tubulin expression. Furthermore, EGFP-Δ3-tubulin expression increased the nuclear size in a dose-dependent manner of EGFP-Δ3-tubulin expression. The expression of EGFP-Δ3-tubulin tended to slow down cell proliferation. Taken together, our results demonstrate that Δ3-tubulin affects the spindle integrity and cell division.
|
| 巻・号 |
57(1)
|
| ページ |
59-67
|
| 公開日 |
2024-3-1
|
| DOI |
10.1007/s00795-023-00373-w
|
| PII |
10.1007/s00795-023-00373-w
|
| PMID |
37930423
|
| MeSH |
Glutamates / metabolism
Glutamates / pharmacology
Humans
Microtubules / metabolism
Mitosis
Pancreatic Neoplasms* / genetics
Pancreatic Neoplasms* / metabolism
Spindle Apparatus / genetics
Spindle Apparatus / metabolism
Tubulin* / genetics
Tubulin* / metabolism
|
| IF |
2.429
|
| リソース情報 |
| ヒト・動物細胞 |
PANC-1(RCB2095) |
