RRC ID 77310
Author Hannachi Imen E, Nakamura M, Mie M, Kobatake E.
Title Construction of multifunctional proteins for tissue engineering: epidermal growth factor with collagen binding and cell adhesive activities.
Journal J Biotechnol
Abstract The development of different techniques based on natural and polymeric scaffolds are useful for the design of different biomimetic materials. These approaches, however, require supplementary steps for the chemical or physical modification of the biomaterial. To avoid such steps, in the present study, we constructed a new multifunctional protein that can be easily immobilized onto hydrophobic surfaces, and at the same time helps enhance specific cell adhesion and proliferation onto collagen substrates. A collagen binding domain was fused to a previously constructed protein, which had an epidermal growth factor fused to a hydrophobic peptide that allows for cell adhesion. The new fusion protein, designated fnCBD-ERE-EGF is produced in Escherichia coli, and its abilities to bind to collagen and promote cell proliferation were investigated. fnCBD-ERE-EGF was shown to keep both collagen binding and cell growth-promoting activities comparable to those of the corresponding unfused proteins. The results obtained in this study also suggest the use of a fnCBD-ERE-EGF as an alternative for the design of multifunctional ECM-bound growth factor based materials.
Volume 139(1)
Pages 19-25
Published 2009-1-1
DOI 10.1016/j.jbiotec.2008.09.011
PII S0168-1656(08)02403-6
PMID 18984018
MeSH Biomimetic Materials / metabolism Cell Adhesion Cell Line Cell Proliferation Collagen / metabolism* Elastin / genetics Elastin / metabolism Epidermal Growth Factor / genetics Epidermal Growth Factor / metabolism* Escherichia coli / genetics Humans Hydrophobic and Hydrophilic Interactions Immobilized Proteins / genetics Immobilized Proteins / metabolism* Models, Molecular Oligopeptides / genetics Oligopeptides / metabolism Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism* Tissue Engineering
IF 3.503
Human and Animal Cells A549(RCB0098)