| RRC ID |
77499
|
| 著者 |
Minegishi T, Uesugi Y, Kaneko N, Yoshida W, Sawamoto K, Inagaki N.
|
| タイトル |
Shootin1b Mediates a Mechanical Clutch to Produce Force for Neuronal Migration.
|
| ジャーナル |
Cell Rep
|
| Abstract |
As an essential step for brain morphogenesis, neurons migrate via mechanical interactions with components of their environment such as neighboring cells and the extracellular matrix. However, the molecular mechanism by which neurons exert forces on their environment during migration remains poorly understood. Here, we show that shootin1b is expressed in migrating mouse olfactory interneurons and accumulates at their leading process growth cone. We demonstrate that shootin1b, by binding to cortactin and L1-CAM, couples F-actin retrograde flow and the adhesive substrate as a clutch molecule. Shootin1b-mediated clutch coupling at the growth cone generates traction force on the substrate, thereby promoting leading process extension and subsequent somal translocation of olfactory interneurons. Furthermore, loss of shootin1 causes abnormal positioning of the interneurons and dysgenesis of the olfactory bulb. Our findings indicate that shootin1b plays a key role in force-driven leading process extension, which propels the migration of olfactory interneurons during olfactory bulb formation.
|
| 巻・号 |
25(3)
|
| ページ |
624-639.e6
|
| 公開日 |
2018-10-16
|
| DOI |
10.1016/j.celrep.2018.09.068
|
| PII |
S2211-1247(18)31528-6
|
| PMID |
30332643
|
| MeSH |
Actins / metabolism
Animals
Brain / cytology
Brain / physiology*
Cell Adhesion
Cell Movement*
Cells, Cultured
Female
Growth Cones / physiology*
Interneurons / cytology
Interneurons / physiology*
Male
Mice
Mice, Inbred C57BL
Mice, Inbred ICR
Mice, Knockout
Nerve Tissue Proteins / physiology*
Olfactory Bulb / cytology
Olfactory Bulb / physiology*
Rats, Wistar
|
| IF |
8.109
|
| リソース情報 |
| 遺伝子材料 |
pGEX-shootin1b (RDB19937)
pCMV-myc-shootin1b (RDB19938) |
