論文 - 詳細
RRC ID | 77514 |
---|---|
著者 | Wu C, Kannan S, Verma CS, Swaminathan K, Wong SM. |
タイトル | Molecular modeling and interaction between Arabidopsis sulfite oxidase and the GW motif of Turnip crinkle virus coat protein. |
ジャーナル | Virology |
Abstract |
Previous study has shown that Hibiscus sulfite oxidase (SO) interacts with Hibiscus chlorotic ringspot virus (HCRSV) coat protein (CP) and triggers sulfur enhanced defense (SED). In this study, we show the interaction of Arabidopsis SO (AtSO) and Turnip crinkle virus (TCV) CP in Arabidopsis thaliana plants. We identified the binding sites of TCV CP (W274) and AtSO (D223) using bioinformatics and confirmed it experimentally. Mutation of binding site W274 to A274 in TCV CP resulted in failure of TCV infection. TCV accumulation in SO over-expression (SO_OE) plants was lower than that in wild-type (WT) and SO knock-out (SO_KO) plants at 7 dpi but reached a level similar to that of WT and SO_KO plants at 10 dpi. AtSO competed with Argonaute 1 (AGO1) for TCV CP binding in vitro. AtSO may serve as an anti-viral factor through sequestering TCV CP for binding with AGO1 and confers virus resistance. |
巻・号 | 551 |
ページ | 64-74 |
公開日 | 2020-12-1 |
DOI | 10.1016/j.virol.2020.08.013 |
PII | S0042-6822(20)30174-4 |
PMID | 33038689 |
MeSH | Arabidopsis* / enzymology Arabidopsis* / virology Arabidopsis Proteins / metabolism* Argonaute Proteins / metabolism Capsid Proteins / metabolism* Carmovirus / metabolism* Host Microbial Interactions Models, Molecular Plant Diseases / virology* Protein Binding Protein Domains Sulfite Oxidase / metabolism* |
IF | 2.819 |
リソース情報 | |
シロイヌナズナ / 植物培養細胞・遺伝子 | pdx02732 |