RRC ID 77515
Author Cajero-Sanchez W, Aceves-Garcia P, Fernández-Marcos M, Gutiérrez C, Rosas U, García-Ponce B, Álvarez-Buylla ER, Sánchez MP, Garay-Arroyo A.
Title Natural Root Cellular Variation in Responses to Osmotic Stress in Arabidopsis thaliana Accessions.
Journal Genes (Basel)
Abstract Arabidopsis naturally occurring populations have allowed for the identification of considerable genetic variation remodeled by adaptation to different environments and stress conditions. Water is a key resource that limits plant growth, and its availability is initially sensed by root tissues. The root's ability to adjust its physiology and morphology under water deficit makes this organ a useful model to understand how plants respond to water stress. Here, we used hyperosmotic shock stress treatments in different Arabidopsis accessions to analyze the root cell morphological responses. We found that osmotic stress conditions reduced root growth and root apical meristem (RAM) size, promoting premature cell differentiation without affecting the stem cell niche morphology. This phenotype was accompanied by a cluster of small epidermal and cortex cells with radial expansion and root hairs at the transition to the elongation zone. We also found this radial expansion with root hairs when plants are grown under hypoosmotic conditions. Finally, root growth was less affected by osmotic stress in the Sg-2 accession followed by Ws, Cvi-0, and Col-0; however, after a strong osmotic stress, Sg-2 and Cvi-0 were the most resilience accessions. The sensitivity differences among these accessions were not explained by stress-related gene expression. This work provides new cellular insights on the Arabidopsis root phenotypic variability and plasticity to osmotic stress.
Volume 10(12)
Published 2019-11-29
DOI 10.3390/genes10120983
PII genes10120983
PMID 31795411
PMC PMC6969899
MeSH Arabidopsis / classification* Arabidopsis / cytology Arabidopsis / genetics Arabidopsis / growth & development* Arabidopsis Proteins / genetics* Cell Differentiation Gene Expression Profiling Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Osmotic Pressure Plant Roots / cytology Plant Roots / genetics Plant Roots / growth & development* Stem Cell Niche Stress, Physiological
IF 3.759
Arabidopsis / Cultured plant cells, genes sja02900 sja04400 sja21500 sja21600 sja25100 sja26400 sja26800 sja33200