論文 - 詳細
| RRC ID | 77525 |
|---|---|
| 著者 | Alvi E, Mochizuki AL, Katsuki Y, Ogawa M, Qi F, Okamoto Y, Takata M, Mu A. |
| タイトル | Mouse Slfn8 and Slfn9 genes complement human cells lacking SLFN11 during the replication stress response. |
| ジャーナル | Commun Biol |
| Abstract |
The Schlafen (SLFN)11 gene has been implicated in various biological processes such as suppression of HIV replication, replication stress response, and sensitization of cancer cells to chemotherapy. Due to the rapid diversification of the SLFN family members, it remains uncertain whether a direct ortholog of human SLFN11 exists in mice. Here we show that mSLFN8/9 and hSLFN11 were rapidly recruited to microlaser-irradiated DNA damage tracks. Furthermore, Slfn8/9 expression could complement SLFN11 loss in human SLFN11-/- cells, and as a result, reduced the growth rate to wild-type levels and partially restored sensitivity to DNA-damaging agents. In addition, both Slfn8/9 and SLFN11 expression accelerated stalled fork degradation and decreased RPA and RAD51 foci numbers after DNA damage. Based on these results, we propose that mouse Slfn8 and Slfn9 genes may share an orthologous function with human SLFN11. This notion may facilitate understanding of SLFN11's biological role through in vivo studies via mouse modeling. |
| 巻・号 | 6(1) |
| ページ | 1038 |
| 公開日 | 2023-10-13 |
| DOI | 10.1038/s42003-023-05406-9 |
| PII | 10.1038/s42003-023-05406-9 |
| PMID | 37833372 |
| PMC | PMC10575959 |
| MeSH | Animals DNA Damage* Endoribonucleases* / genetics Humans Mice Nuclear Proteins* / metabolism |
| IF | 4.165 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | CSIV-TRE-RfA-UbC-KT (RDB12878) pENTR-hSLFN5 (RDB20418) pENTR-hSLFN13 (RDB20419) pENTR-mSlfn8 (RDB20420) pENTR-mSlfn9 (RDB20421) pcDNA3-GFP-mSlfn8 (RDB20422) pcDNA3-GFP-mSlfn9 (RDB20423) pcDNA3-GFP-hSLFN5 (RDB20424) pcDNA3-GFP-hSLFN13 (RDB20425) pcDNA3-GFP-hSLFN11 (RDB20426) |