論文 - 詳細
| RRC ID | 77545 |
|---|---|
| 著者 | Yao K, Zhang L, Zhang Y, Ye P, Zhu N. |
| タイトル | The flavonoid, fisetin, inhibits UV radiation-induced oxidative stress and the activation of NF-kappaB and MAPK signaling in human lens epithelial cells. |
| ジャーナル | Mol Vis |
| Abstract |
PURPOSE:Ultraviolet (UV) radiation-induced oxidative stress plays a significant role in the progression of cataracts. This study investigated the photoprotective effect of fisetin on UV radiation-induced oxidative stress in human lens epithelial cells and the possible molecular mechanism involved. METHODS:SRA01/04 cells exposed to different doses of ultraviolet B (UVB) were cultured with various concentrations of fisetin and subsequently monitored for cell viability by the 4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT) assay. The effect of fisetin on the generation of reactive oxygen species (ROS) of SRA01/04 cells was determined by flow cytometry. Translocation of nuclear factor kappa-B (NF-kappaB) was examined by immunocytochemistry. Expression of NF-kappaB/P65, inhibiter kappa B (IkappaB), and mitogen activated protein kinase (MAPK) proteins were measured by western blot. RESULTS:Treatment of SRA01/04 cells with fisetin inhibited UVB-induced cell death and the generation of ROS. Fisetin inhibited UVB-induced activation and translocation of NF-kappaB/p65, which was mediated through an inhibition of the degradation and activation of IkappaB. Fisetin also inhibited UVB-induced phosphorylation of the p38 and c-Jun N-terminal kinase (JNK) proteins of the MAPK family at various time points studied. CONCLUSIONS:The flavonoid, fisetin, could be useful in attenuation of UV radiation-induced oxidative stress and the activation of NF-kappaB and MAPK signaling in human lens epithelial cells, which suggests that fisetin has a potential protective effect against cataractogenesis. |
| 巻・号 | 14 |
| ページ | 1865-71 |
| 公開日 | 2008-1-1 |
| PMID | 18949064 |
| PMC | PMC2571947 |
| MeSH | Cell Line Cell Survival / drug effects Cell Survival / radiation effects Epithelial Cells / cytology Epithelial Cells / drug effects Epithelial Cells / enzymology* Epithelial Cells / radiation effects Flavonoids / pharmacology* Flavonols Humans I-kappa B Proteins / metabolism Lens, Crystalline / cytology* MAP Kinase Signaling System / drug effects* MAP Kinase Signaling System / radiation effects Mitogen-Activated Protein Kinases / metabolism NF-kappa B / metabolism* Oxidative Stress / drug effects* Oxidative Stress / radiation effects Phosphorylation / drug effects Protein Processing, Post-Translational / drug effects Protein Transport / drug effects Protein Transport / radiation effects Reactive Oxygen Species / metabolism Ultraviolet Rays* |
| IF | 2.202 |
| リソース情報 | |
| ヒト・動物細胞 | SRA 01/04(RCB1591) |