Reference - Detail
RRC ID | 77828 |
---|---|
Author | Takenobu T, Tomizawa K, Matsushita M, Li ST, Moriwaki A, Lu YF, Matsui H. |
Title | Development of p53 protein transduction therapy using membrane-permeable peptides and the application to oral cancer cells. |
Journal | Mol Cancer Ther |
Abstract |
Recent studies suggest that several proteins can transverse biological membranes through protein transduction. The protein transduction domains of these proteins, 10-16 residues long, have been identified as critical domains for the protein transduction. Poly-arginine peptide also has the ability of protein transduction. Here, we show that the protein delivery system using 11 poly-arginine peptides (11R) is a powerful tool for the transduction of the biologically active tumor suppressor protein, p53, to suppress the proliferation of oral cancer cells. The 11R-fused p53 proteins (11R-p53) effectively penetrated across the plasma membrane of the cancer cells and translocated into the nucleus. The proteins induced the activity of the p21/WAF promoter and inhibited the proliferation of human oral cancer cells, in which the p53 gene was mutated. The effect was equivalent to that of the adenovirus-mediated p53 gene transduction system. Moreover, 11R-p53 enhanced the cisplatin-dependent induction of apoptosis of the cells. These data suggest that this protein transduction method may become a promising cancer therapy. |
Volume | 1(12) |
Pages | 1043-9 |
Published | 2002-10-1 |
PMID | 12481427 |
MeSH | Active Transport, Cell Nucleus Adenoviridae / genetics Antineoplastic Agents / pharmacology Apoptosis Blotting, Western Cell Division Cell Membrane / metabolism Cell Nucleus / metabolism Cell Survival Cisplatin / pharmacology Cyclin-Dependent Kinase Inhibitor p21 Cyclins / metabolism Gene Transfer Techniques* Genes, Reporter Genetic Vectors Humans Immunohistochemistry Microscopy, Fluorescence Mouth Neoplasms / genetics* Mouth Neoplasms / metabolism Mouth Neoplasms / therapy* Peptides / chemistry Plasmids / metabolism Protein Structure, Tertiary Recombinant Fusion Proteins / metabolism Time Factors Tumor Suppressor Protein p53 / chemistry Tumor Suppressor Protein p53 / metabolism* |
IF | 5.615 |
Resource | |
Human and Animal Cells | Saos-2(RCB0428) |