論文 - 詳細
| RRC ID | 78051 |
|---|---|
| 著者 | Takado M, Yamamoto TG, Chikashige Y, Matsumoto T. |
| タイトル | Fission yeast Wee1 is required for stable kinetochore-microtubule attachment. |
| ジャーナル | Open Biol |
| Abstract |
Wee1 is a cell cycle regulator that phosphorylates Cdk1/Cdc2 and inhibits G2/M transition. Loss of Wee1 in fission yeast results in an early onset of mitosis. Interestingly, we found that cells lacking Wee1 require the functional spindle checkpoint for their viability. Genetic analysis indicated that the requirement is not attributable to the early onset of mitosis. Live-cell imaging revealed that some kinetochores are not attached or bioriented in the wee1 mutant. Furthermore, Mad2, a component of the spindle checkpoint known to recognize unattached kinetochores, accumulates in the vicinity of the spindle, representing activation of the spindle checkpoint in the mutant. It appears that the wee1 mutant cannot maintain stable kinetochore-microtubule attachment, and relies on the delay imposed by the spindle checkpoint for establishing biorientation of kinetochores. This study revealed a role of Wee1 in ensuring accurate segregation of chromosomes during mitosis, and thus provided a basis for a new principle of cancer treatment with Wee1 inhibitors. |
| 巻・号 | 14(1) |
| ページ | 230379 |
| 公開日 | 2024-1-1 |
| DOI | 10.1098/rsob.230379 |
| PMID | 38166399 |
| PMC | PMC10762435 |
| MeSH | Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism Kinetochores / metabolism Microtubules / metabolism Mitosis Nuclear Proteins / genetics Nuclear Proteins / metabolism Protein-Tyrosine Kinases / genetics Protein-Tyrosine Kinases / metabolism Schizosaccharomyces* / genetics Schizosaccharomyces* / metabolism Schizosaccharomyces pombe Proteins* / genetics Schizosaccharomyces pombe Proteins* / metabolism Spindle Apparatus / metabolism |
| IF | 4.931 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | |