| RRC ID |
78066
|
| 著者 |
Shibagaki N, Hanada Ki, Yamashita H, Shimada S, Hamada H.
|
| タイトル |
Overexpression of CD82 on human T cells enhances LFA-1 / ICAM-1-mediated cell-cell adhesion: functional association between CD82 and LFA-1 in T cell activation.
|
| ジャーナル |
Eur J Immunol
|
| Abstract |
We previously demonstrated that CD82, expressed on both T cells and antigen-presenting cells (APC), plays an important role as a co-stimulatory molecule especially in the early phase of T cell activation. We also showed that the CD82 expression level is up-regulated on activated T cells and memory T cells. This up-regulation enhances both T cell-T cell and T cell-APC interactions. In this study, we further investigated the mechanism of CD82-mediated cell-cell adhesion. The enhanced adhesion between CD82-overexpressing Jurkat cells was completely blocked by anti-ICAM-1 / LFA-1 monoclonal antibodies. Increased interaction of LFA-1 with ICAM-1 was further confirmed by enhanced adhesion of CD82-overexpressing Jurkat cells to immobilized ICAM-1-Ig. CD82 co-immunoprecipitated with LFA-1 from Jurkat cells and CD82 and LFA-1 colocalized at an adhesion foci. These results suggest that the T cell stimulation via anti-CD3 cross-linking or phorbol myristate acetate treatment up-regulates CD82 expression, leading to the colocalization of CD82 and LFA-1, and results in enhanced interaction between LFA-1 and ICAM-1. In addition, a blocking experiment using monoclonal antibodies suggested that CD82 and LFA-1 molecules on APC are also important for the optimal activation of T cells. This is the first report that describes the enhancement of cell-cell interaction through LFA-1 and ICAM-1 by the overexpression of another cell surface molecule, CD82.
|
| 巻・号 |
29(12)
|
| ページ |
4081-91
|
| 公開日 |
1999-12-1
|
| DOI |
10.1002/(SICI)1521-4141(199912)29:12<4081::AID-IMMU4081>3.0.CO;2-I
|
| PMID |
10602019
|
| MeSH |
Antigens, CD / biosynthesis
Antigens, CD / immunology*
Cell Adhesion / immunology
Cell Communication / immunology
Cells, Cultured
Humans
Intercellular Adhesion Molecule-1 / immunology*
Kangai-1 Protein
Lymphocyte Activation
Lymphocyte Function-Associated Antigen-1 / immunology*
Membrane Glycoproteins / biosynthesis
Membrane Glycoproteins / immunology*
Proto-Oncogene Proteins*
Signal Transduction / immunology
T-Lymphocytes / cytology*
T-Lymphocytes / immunology*
|
| リソース情報 |
| ヒト・動物細胞 |
Meth-A(RCB0464) |
