論文 - 詳細
| RRC ID | 78066 |
|---|---|
| 著者 | Shibagaki N, Hanada Ki, Yamashita H, Shimada S, Hamada H. |
| タイトル | Overexpression of CD82 on human T cells enhances LFA-1 / ICAM-1-mediated cell-cell adhesion: functional association between CD82 and LFA-1 in T cell activation. |
| ジャーナル | Eur J Immunol |
| Abstract |
We previously demonstrated that CD82, expressed on both T cells and antigen-presenting cells (APC), plays an important role as a co-stimulatory molecule especially in the early phase of T cell activation. We also showed that the CD82 expression level is up-regulated on activated T cells and memory T cells. This up-regulation enhances both T cell-T cell and T cell-APC interactions. In this study, we further investigated the mechanism of CD82-mediated cell-cell adhesion. The enhanced adhesion between CD82-overexpressing Jurkat cells was completely blocked by anti-ICAM-1 / LFA-1 monoclonal antibodies. Increased interaction of LFA-1 with ICAM-1 was further confirmed by enhanced adhesion of CD82-overexpressing Jurkat cells to immobilized ICAM-1-Ig. CD82 co-immunoprecipitated with LFA-1 from Jurkat cells and CD82 and LFA-1 colocalized at an adhesion foci. These results suggest that the T cell stimulation via anti-CD3 cross-linking or phorbol myristate acetate treatment up-regulates CD82 expression, leading to the colocalization of CD82 and LFA-1, and results in enhanced interaction between LFA-1 and ICAM-1. In addition, a blocking experiment using monoclonal antibodies suggested that CD82 and LFA-1 molecules on APC are also important for the optimal activation of T cells. This is the first report that describes the enhancement of cell-cell interaction through LFA-1 and ICAM-1 by the overexpression of another cell surface molecule, CD82. |
| 巻・号 | 29(12) |
| ページ | 4081-91 |
| 公開日 | 1999-12-1 |
| DOI | 10.1002/(SICI)1521-4141(199912)29:12<4081::AID-IMMU4081>3.0.CO;2-I |
| PMID | 10602019 |
| MeSH | Antigens, CD / biosynthesis Antigens, CD / immunology* Cell Adhesion / immunology Cell Communication / immunology Cells, Cultured Humans Intercellular Adhesion Molecule-1 / immunology* Kangai-1 Protein Lymphocyte Activation Lymphocyte Function-Associated Antigen-1 / immunology* Membrane Glycoproteins / biosynthesis Membrane Glycoproteins / immunology* Proto-Oncogene Proteins* Signal Transduction / immunology T-Lymphocytes / cytology* T-Lymphocytes / immunology* |
| IF | 4.404 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Meth-A(RCB0464) |