RRC ID 78624
Author Takiguchi K, Shimizu H, Shoda K, Shiraishi K, Furuya S, Hosomura N, Akaike H, Kawaguchi Y, Amemiya H, Kawaida H, Mochizuki K, Kondo T, Ichikawa D.
Title The Expression and Role of NADPH Oxidase 2 in Colon Cancer.
Journal Anticancer Res
Abstract BACKGROUND/AIM:Recent studies have reported that nicotinamide adenine dinucleotide phosphate oxidases (NOXs) are expressed in various cancers and play important roles in tumor progression. However, no studies have examined the expression and role of NOX2 in colon cancer. The aim of this study is to investigate the pathophysiological roles of NOX2 in colon cancer patients and cell lines.
PATIENTS AND METHODS:One-hundred and sixteen primary colon cancer samples of patients who underwent radical resection for locally advanced colon cancer were used for immunohistochemistry of NOX2 protein. The relationship between NOX2 expression and clinicopathological factors was assessed and the prognostic significance of NOX2 expression was evaluated in colon cancer patients. NOX2 siRNA transfection experiments were performed using two colon cancer cell lines (HCT116 and RKO) to analyze the impact of NOX2 expression on cellular physiological functions.
RESULTS:The expression of NOX2 protein in noncancerous tissue was scarcely observed, and 45 samples (38.8%) showed positively stained NOX2 expression in cancer tissue. There were no clinicopathological factors significantly associated with NOX2 expression. The 5-year recurrence-free survival rate of the NOX2 positive group was significantly lower than that of the NOX2 negative group (61.1% vs. 79.3%, p=0.029). NOX2 depletion significantly inhibited cell proliferation with G1 arrest, and motility in the two cell lines.
CONCLUSION:NOX2 expression level has a close association with the prognosis of colon cancer patients and physiological functions of colon cancer cells. NOX2 may be a useful prognostic biomarker for colon cancer patients.
Volume 43(6)
Pages 2601-2608
Published 2023-6-1
DOI 10.21873/anticanres.16427
PII 43/6/2601
PMID 37247898
MeSH Colonic Neoplasms* / genetics Colonic Neoplasms* / pathology Humans NADPH Oxidase 2 / genetics NADPH Oxidase 2 / metabolism NADPH Oxidases* / genetics NADPH Oxidases* / metabolism Prognosis Reactive Oxygen Species / metabolism
Human and Animal Cells HCT116(RCB2979)