論文 - 詳細
| RRC ID | 78651 |
|---|---|
| 著者 | Kotani N, Araki T, Miyagawa-Yamaguchi A, Amimoto T, Nakano M, Honke K. |
| タイトル | Proximity Labeling and Proteomics: Get to Know Neighbors. |
| ジャーナル | Methods Enzymol |
| Abstract |
Protein-protein interactions are essential in biological reactions and fundamental to cell-cell communication (e.g., the binding of secreted proteins, such as hormones, to cell membrane receptors) and the subsequent intracellular signal transduction cascade. Several studies have been extensively carried out on protein-protein interactions because they have the potential to resolve various problems in molecular biology. Biochemical methods, such as chemical cross-linking and immunoprecipitation, have long been used to analyze which proteins interact with each other. However, there are some problems, such as unphysiological states and non-specific binding, that require the development of more useful experimental methods. This chapter discusses the "proximity labeling (Proteomics)" analysis technique, which has been attracting attention in protein-protein interaction analysis in recent years and is used in many biological studies. "Membrane proximity labeling (proteomics)," which analyzes the interaction of cell membrane proteins, and "intracellular proximity labeling (proteomics)" will be explained in-depth. |
| 巻・号 | 679 |
| ページ | 131-162 |
| 公開日 | 2023-1-1 |
| DOI | 10.1016/bs.mie.2022.07.031 |
| PII | S0076-6879(22)00295-6 |
| PMID | 36682860 |
| MeSH | Cell Membrane / metabolism Membrane Proteins* / metabolism Proteomics* / methods Staining and Labeling |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | LK-2(RCB1970) |