RRC ID 79049
Author Yoshida K, Hayashi S.
Title Epidermal growth factor receptor signaling protects epithelia from morphogenetic instability and tissue damage in Drosophila.
Journal Development
Abstract Dying cells in the epithelia communicate with neighboring cells to initiate coordinated cell removal to maintain epithelial integrity. Naturally occurring apoptotic cells are mostly extruded basally and engulfed by macrophages. Here, we have investigated the role of Epidermal growth factor (EGF) receptor (EGFR) signaling in the maintenance of epithelial homeostasis. In Drosophila embryos, epithelial tissues undergoing groove formation preferentially enhanced extracellular signal-regulated kinase (ERK) signaling. In EGFR mutant embryos at stage 11, sporadic apical cell extrusion in the head initiates a cascade of apical extrusions of apoptotic and non-apoptotic cells that sweeps the entire ventral body wall. Here, we show that this process is apoptosis dependent, and clustered apoptosis, groove formation, and wounding sensitize EGFR mutant epithelia to initiate massive tissue disintegration. We further show that tissue detachment from the vitelline membrane, which frequently occurs during morphogenetic processes, is a key trigger for the EGFR mutant phenotype. These findings indicate that, in addition to cell survival, EGFR plays a role in maintaining epithelial integrity, which is essential for protecting tissues from transient instability caused by morphogenetic movement and damage.
Volume 150(5)
Published 2023-3-1
DOI 10.1242/dev.201231
PII 297057
PMID 36897356
PMC PMC10108703
MeSH Animals Drosophila* / metabolism Epidermal Growth Factor / metabolism Epithelium / metabolism ErbB Receptors* / metabolism Extracellular Signal-Regulated MAP Kinases / metabolism Phosphorylation Signal Transduction*
Drosophila DGRC#106395 DGRC#118527