| RRC ID |
79475
|
| 著者 |
Tsukamoto A, Higashiyama S, Yoshida K, Watanabe Y, Furukawa KS, Ushida T.
|
| タイトル |
Stable cavitation induces increased cytoplasmic calcium in L929 fibroblasts exposed to 1-MHz pulsed ultrasound.
|
| ジャーナル |
Ultrasonics
|
| Abstract |
An increase in cytoplasmic calcium (Ca(2+) increase) is a second messenger that is often observed under ultrasound irradiation. We hypothesize that cavitation is a physical mechanism that underlies the increase in Ca(2+) in these experiments. To control the presence of cavitation, the wave type was controlled in a sonication chamber. One wave type largely contained a traveling wave (wave type A) while the other wave type largely contained a standing wave (wave type B). Fast Fourier transform (FFT) analysis of a sound field produced by the wave types ascertained that stable cavitation was present only under wave type A ultrasound irradiation. Under the two controlled wave types, the increase in Ca(2+) in L929 fibroblasts was observed with fluorescence imaging. Under wave type A ultrasound irradiation, an increase in Ca(2+) was observed; however, no increase in Ca(2+) was observed under wave type B ultrasound irradiation. We conclude that stable cavitation is involved in the increase of Ca(2+) in cells subjected to pulsed ultrasound.
|
| 巻・号 |
51(8)
|
| ページ |
982-90
|
| 公開日 |
2011-12-1
|
| DOI |
10.1016/j.ultras.2011.05.014
|
| PII |
S0041-624X(11)00101-6
|
| PMID |
21689836
|
| MeSH |
Animals
Calcium / metabolism*
Cells, Cultured
Cytosol / diagnostic imaging*
Cytosol / metabolism*
Equipment Design
Fibroblasts / diagnostic imaging*
Fibroblasts / metabolism*
Mice
Sonication / methods*
Transducers
Ultrasonography
|
| リソース情報 |
| ヒト・動物細胞 |
L929(RCB1451) |
