RRC ID 80417
Author Liang Y, Lu ZM, Shi W, Wu LH, Chai LJ, Zhang XJ, Zhang SY, Wang ST, Shen CH, Xu ZH.
Title Lentibacillus daqui sp. nov., isolated from high-temperature Daqu, a starter for production of Chinese Jiang-flavour Baijiu.
Journal Int J Syst Evol Microbiol
Abstract A Gram-stain-positive, rod-shaped, endospore-forming, aerobic bacterial strain, designated ZS110521T, was isolated from high-temperature Daqu, a starter for production of Chinese Jiang-flavour Baijiu and was characterised by polyphasic taxonomy. This novel isolate grew in the presence of 0-20 % (w/v) NaCl, at pH 6.0-9.0 and 20-50 °C; optimum growth was observed with 8-10 % (w/v) NaCl, at pH 7.0 and 37 °C. A comparative analysis of the 16S rRNA gene sequence (1460 bp) of ZS110521T revealed that it displayed the highest similarity to Lentibacillus populi WD4L-1T (95.5 %), followed by Lentibacillus garicola SL-MJ1T (95.4 %) and Lentibacillus lacisalsi BH260T (95.2 %). ANI and dDDH values between ZS110521T and other strains of species of the genus Lentibacillus were less than 78 and 28 %, respectively. The predominant cellular fatty acids (> 10 %) of ZS110521T were anteiso-C17 : 0 (37.8 %), anteiso-C15 : 0 (28.1 %) and iso-C16 : 0 (15.5 %). The respiratory quinone was identified as menaquinone-7 (MK-7) and the major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The polyphasic taxonomic data and the results of chemotaxonomic analysis confirmed that ZS110521T represents a novel species, for which the name Lentibacillus daqui sp. nov. is proposed. The type strain of this proposed species is ZS110521T (=CGMCC 1.19456T =JCM 35213T).
Volume 73(8)
Published 2023-8-1
DOI 10.1099/ijsem.0.005962
PMID 37534981
MeSH Alcoholic Beverages* / microbiology Bacillaceae* / classification Bacillaceae* / isolation & purification Bacterial Typing Techniques Base Composition DNA, Bacterial / genetics Fatty Acids / chemistry Phospholipids / chemistry Phylogeny* RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Temperature
General Microbes JCM 35213 JCM 30131