論文 - 詳細
| RRC ID | 80709 |
|---|---|
| 著者 | Bat-Erdene Jargalsaikhan, Masanaga Muto, Youngeun Been, Shoma Matsumoto, Eiichi Okamura, Tadanobu Takahashi, Yutaka Narimichi, Yuuki Kurebayashi, Hideyuki Takeuchi, Takashi Shinohara, Ryo Yamamoto, Masatsugu Ema |
| タイトル | The Dual-Pseudotyped Lentiviral Vector with VSV-G and Sendai Virus HN Enhances Infection Efficiency through the Synergistic Effect of the Envelope Proteins |
| ジャーナル | Viruses |
| Abstract |
A gene delivery system utilizing lentiviral vectors (LVs) requires high transduction efficiency for successful application in human gene therapy. Pseudotyping allows viral tropism to be expanded, widening the usage of LVs. While vesicular stomatitis virus G (VSV-G) single-pseudotyped LVs are commonly used, dual-pseudotyping is less frequently employed because of its increased complexity. In this study, we examined the potential of phenotypically mixed heterologous dual-pseudotyped LVs with VSV-G and Sendai virus hemagglutinin-neuraminidase (SeV-HN) glycoproteins, termed V/HN-LV. Our findings demonstrated the significantly improved transduction efficiency of V/HN-LV in various cell lines of mice, cynomolgus monkeys, and humans compared with LV pseudotyped with VSV-G alone. Notably, V/HN-LV showed higher transduction efficiency in human cells, including hematopoietic stem cells. The efficient incorporation of wild-type SeV-HN into V/HN-LV depended on VSV-G. SeV-HN removed sialic acid from VSV-G, and the desialylation of VSV-G increased V/HN-LV infectivity. Furthermore, V/HN-LV acquired the ability to recognize sialic acid, particularly N-acetylneuraminic acid on the host cell, enhancing LV infectivity. Overall, VSV-G and SeV-HN synergistically improve LV transduction efficiency and broaden its tropism, indicating their potential use in gene delivery. |
| 巻・号 | 16 |
| ページ | 827 |
| 公開日 | 2024-5-23 |
| DOI | 10.3390/v16060827 |
| PMID | 38932120 |
| PMC | PMC11209056 |
| MeSH | Animals Cell Line Gene Transfer Techniques Genetic Therapy / methods Genetic Vectors* / genetics HEK293 Cells HN Protein* / genetics HN Protein* / metabolism Humans Lentivirus* / genetics Macaca fascicularis Membrane Glycoproteins / genetics Membrane Glycoproteins / metabolism Mice Sendai virus* / genetics Transduction, Genetic* Viral Envelope Proteins* / genetics Viral Envelope Proteins* / metabolism Viral Tropism |
| IF | 3.816 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | CS-CA-GFP (RDB05964) pCAG-HIVgp (RDB04394) pCMV-VSV-G (RDB04392) pCMV-VSV-G-RSV-Rev (RDB04393) |