論文 - 詳細
| RRC ID | 80829 |
|---|---|
| 著者 | Chen S, Phillips CM. |
| タイトル | HRDE-2 drives small RNA specificity for the nuclear Argonaute protein HRDE-1. |
| ジャーナル | Nat Commun |
| Abstract |
RNA interference (RNAi) is a conserved gene silencing process that exists in diverse organisms to protect genome integrity and regulate gene expression. In C. elegans, the majority of RNAi pathway proteins localize to perinuclear, phase-separated germ granules, which are comprised of sub-domains referred to as P granules, Mutator foci, Z granules, and SIMR foci. However, the protein components and function of the newly discovered SIMR foci are unknown. Here we demonstrate that HRDE-2 localizes to SIMR foci and interacts with the germline nuclear Argonaute HRDE-1 in its small RNA unbound state. In the absence of HRDE-2, HRDE-1 exclusively loads CSR-class 22G-RNAs rather than WAGO-class 22G-RNAs, resulting in inappropriate H3K9me3 deposition on CSR-target genes. Thus, our study demonstrates that the recruitment of unloaded HRDE-1 to germ granules, mediated by HRDE-2, is critical to ensure that the correct small RNAs are used to guide nuclear RNA silencing in the C. elegans germline. |
| 巻・号 | 15(1) |
| ページ | 957 |
| 公開日 | 2024-2-1 |
| DOI | 10.1038/s41467-024-45245-8 |
| PII | 10.1038/s41467-024-45245-8 |
| PMID | 38302462 |
| PMC | PMC10834429 |
| MeSH | Animals Argonaute Proteins / genetics Argonaute Proteins / metabolism Caenorhabditis elegans* / genetics Caenorhabditis elegans* / metabolism Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism Germ Cells / metabolism RNA Interference RNA, Small Interfering / genetics RNA, Small Interfering / metabolism |
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オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1200 tm116 tm116102 |