RRC ID 80976
Author Kobayashi T, Yamashita A, Tsumaki N, Watanabe H.
Title Subpopulations of fibroblasts derived from human iPS cells.
Journal Commun Biol
Abstract Organ fibrosis causes collagen fiber overgrowth and impairs organ function. Cardiac fibrosis after myocardial infarction impairs cardiac function significantly, pulmonary fibrosis reduces gas exchange efficiency, and liver fibrosis disturbs the natural function of the liver. Its development is associated with the differentiation of fibroblasts into myofibroblasts and increased collagen synthesis. Fibrosis has organ specificity, defined by the heterogeneity of fibroblasts. Although this heterogeneity is established during embryonic development, it has not been defined yet. Fibroblastic differentiation of induced pluripotent stem cells (iPSCs) recapitulates the process by which fibroblasts acquire diversity. Here, we differentiated iPSCs into cardiac, hepatic, and dermal fibroblasts and analyzed their properties using single-cell RNA sequencing. We observed characteristic subpopulations with different ratios in each organ-type fibroblast group, which contained both resting and distinct ACTA2+ myofibroblasts. These findings provide crucial information on the ontogeny-based heterogeneity of fibroblasts, leading to the development of therapeutic strategies to control fibrosis.
Volume 7(1)
Pages 736
Published 2024-6-18
DOI 10.1038/s42003-024-06419-8
PII 10.1038/s42003-024-06419-8
PMID 38890483
MeSH Cell Differentiation* Cells, Cultured Fibroblasts* / metabolism Fibrosis Humans Induced Pluripotent Stem Cells* / cytology Induced Pluripotent Stem Cells* / metabolism Myofibroblasts / cytology Myofibroblasts / metabolism Single-Cell Analysis
Human and Animal Cells 1383D2(HPS1005)