Reference - Detail
RRC ID | 81170 |
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Author | Egami Y, Kawai K, Araki N. |
Title | Rit1-TBC1D10B signaling modulates FcγR-mediated phagosome formation in RAW264 macrophages. |
Journal | Life Sci Alliance |
Abstract |
Phagocytosis is an important immune response that protects the host from pathogen invasion. Rit1 GTPase is known to be involved in diverse cellular processes. However, its role in FcγR-mediated phagocytosis remains unclear. Our live-cell imaging analysis revealed that Rit1 was localized to the membranes of F-actin-rich phagocytic cups in RAW264 macrophages. Rit1 knockout and expression of the GDP-locked Rit1 mutant suppressed phagosome formation. We also found that TBC1D10B, a GAP for the Rab family GTPases, colocalizes with Rit1 in the membranes of phagocytic cups. Expression and knockout studies have shown that TBC1D10B decreases phagosome formation in both Rab-GAP activity-dependent and -independent manners. Notably, the expression of the GDP-locked Rit1 mutant or Rit1 knockout inhibited the dissociation of TBC1D10B from phagocytic cups. In addition, the expression of the GTP-locked Rit1 mutant promoted the dissociation of TBC1D10B in phagocytic cups and restored the rate of phagosome formation in TBC1D10B-expressing cells. These data suggest that Rit1-TBC1D10B signaling regulates FcγR-mediated phagosome formation in macrophages. |
Volume | 7(10) |
Published | 2024-10-1 |
DOI | 10.26508/lsa.202402651 |
PII | 7/10/e202402651 |
PMID | 39084876 |
PMC | PMC11291910 |
MeSH | Actins / metabolism Animals GTPase-Activating Proteins* / genetics GTPase-Activating Proteins* / metabolism Macrophages* / metabolism Mice Phagocytosis* / genetics Phagosomes* / metabolism RAW 264.7 Cells Receptors, IgG* / metabolism Signal Transduction* rab GTP-Binding Proteins / genetics rab GTP-Binding Proteins / metabolism |
Resource | |
Human and Animal Cells | RAW 264(RCB0535) COS-7(RCB0539) |