RRC ID 81170
Author Egami Y, Kawai K, Araki N.
Title Rit1-TBC1D10B signaling modulates FcγR-mediated phagosome formation in RAW264 macrophages.
Journal Life Sci Alliance
Abstract Phagocytosis is an important immune response that protects the host from pathogen invasion. Rit1 GTPase is known to be involved in diverse cellular processes. However, its role in FcγR-mediated phagocytosis remains unclear. Our live-cell imaging analysis revealed that Rit1 was localized to the membranes of F-actin-rich phagocytic cups in RAW264 macrophages. Rit1 knockout and expression of the GDP-locked Rit1 mutant suppressed phagosome formation. We also found that TBC1D10B, a GAP for the Rab family GTPases, colocalizes with Rit1 in the membranes of phagocytic cups. Expression and knockout studies have shown that TBC1D10B decreases phagosome formation in both Rab-GAP activity-dependent and -independent manners. Notably, the expression of the GDP-locked Rit1 mutant or Rit1 knockout inhibited the dissociation of TBC1D10B from phagocytic cups. In addition, the expression of the GTP-locked Rit1 mutant promoted the dissociation of TBC1D10B in phagocytic cups and restored the rate of phagosome formation in TBC1D10B-expressing cells. These data suggest that Rit1-TBC1D10B signaling regulates FcγR-mediated phagosome formation in macrophages.
Volume 7(10)
Published 2024-10-1
DOI 10.26508/lsa.202402651
PII 7/10/e202402651
PMID 39084876
PMC PMC11291910
MeSH Actins / metabolism Animals GTPase-Activating Proteins* / genetics GTPase-Activating Proteins* / metabolism Macrophages* / metabolism Mice Phagocytosis* / genetics Phagosomes* / metabolism RAW 264.7 Cells Receptors, IgG* / metabolism Signal Transduction* rab GTP-Binding Proteins / genetics rab GTP-Binding Proteins / metabolism
Resource
Human and Animal Cells RAW 264(RCB0535) COS-7(RCB0539)