論文 - 詳細
| RRC ID | 81281 |
|---|---|
| 著者 | Ueno K, Kurazumi H, Suzuki R, Yanagihara M, Mizoguchi T, Harada T, Morikage N, Hamano K. |
| タイトル | miR-709 exerts an angiogenic effect through a FGF2 upregulation induced by a GSK3B downregulation. |
| ジャーナル | Sci Rep |
| Abstract |
The aim of this study was to identify angiogenic microRNAs (miRNAs) that could be used in the treatment of hindlimb ischemic tissues. miRNAs contained in extracellular vesicles (EVs) deriving from the plasma were analyzed in C57BL/6 mice, which have ischemia tolerance, and in BALB/c mice without ischemia tolerance as part of a hindlimb ischemia model; as a result 43 angiogenic miRNA candidates were identified. An aortic ring assay was employed by using femoral arteries isolated from BALC/c mice and EVs containing miRNA; as a result, the angiogenic miRNA candidates were limited to 14. The blood flow recovery was assessed after injecting EVs containing miRNA into BALB/c mice with hindlimb ischemia, and miR-709 was identified as a promising angiogenic miRNA. miR-709-encapsulating EVs were found to increase the expression levels of the fibroblast growth factor 2 (FGF2) mRNA in the thigh tissues of hindlimb ischemia model BALB/c mice. miR-709 was also found to bind to the 3'UTR of glycogen synthase kinase 3 beta (GSK3B) in three places. GSK3B-knockdown human artery-derived endothelial cells were found to express high levels of FGF2, and were characterized by increased cell proliferation. These findings indicate that miR-709 induces an upregulation of FGF2 through the downregulation of GSK3B. |
| 巻・号 | 14(1) |
| ページ | 11372 |
| 公開日 | 2024-5-18 |
| DOI | 10.1038/s41598-024-62340-4 |
| PII | 10.1038/s41598-024-62340-4 |
| PMID | 38762650 |
| PMC | PMC11102560 |
| MeSH | 3' Untranslated Regions Animals Cell Proliferation Disease Models, Animal Down-Regulation Endothelial Cells / metabolism Extracellular Vesicles / metabolism Fibroblast Growth Factor 2* / genetics Fibroblast Growth Factor 2* / metabolism Glycogen Synthase Kinase 3 beta* / genetics Glycogen Synthase Kinase 3 beta* / metabolism Hindlimb* / blood supply Humans Ischemia* / genetics Ischemia* / metabolism Male Mice Mice, Inbred BALB C* Mice, Inbred C57BL MicroRNAs* / genetics MicroRNAs* / metabolism Neovascularization, Physiologic* / genetics Up-Regulation |
| IF | 3.998 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 293T(RCB2202) |