RRC ID 81297
Author Ye W, Munemasa S, Shinya T, Wu W, Ma T, Lu J, Kinoshita T, Kaku H, Shibuya N, Murata Y.
Title Stomatal immunity against fungal invasion comprises not only chitin-induced stomatal closure but also chitosan-induced guard cell death.
Journal Proc Natl Acad Sci U S A
Abstract Many pathogenic fungi exploit stomata as invasion routes, causing destructive diseases of major cereal crops. Intensive interaction is expected to occur between guard cells and fungi. In the present study, we took advantage of well-conserved molecules derived from the fungal cell wall, chitin oligosaccharide (CTOS), and chitosan oligosaccharide (CSOS) to study how guard cells respond to fungal invasion. In Arabidopsis, CTOS induced stomatal closure through a signaling mediated by its receptor CERK1, Ca2+, and a major S-type anion channel, SLAC1. CSOS, which is converted from CTOS by chitin deacetylases from invading fungi, did not induce stomatal closure, suggesting that this conversion is a fungal strategy to evade stomatal closure. At higher concentrations, CSOS but not CTOS induced guard cell death in a manner dependent on Ca2+ but not CERK1. These results suggest that stomatal immunity against fungal invasion comprises not only CTOS-induced stomatal closure but also CSOS-induced guard cell death.
Volume 117(34)
Pages 20932-20942
Published 2020-8-25
DOI 10.1073/pnas.1922319117
PII 1922319117
PMID 32778594
PMC PMC7456093
MeSH Arabidopsis / metabolism Arabidopsis Proteins / metabolism Arabidopsis Proteins / physiology Calcium / metabolism Cell Death / drug effects Chitin / metabolism* Chitin / physiology Chitosan / metabolism Fungi / metabolism Plant Stomata / immunology* Plant Stomata / metabolism* Protein Kinases / metabolism Protein Serine-Threonine Kinases / metabolism Protein Serine-Threonine Kinases / physiology Signal Transduction / drug effects
Resource
Arabidopsis / Cultured plant cells, genes pst14772