RRC ID |
81355
|
Author |
Lleras Forero L, Narayanan R, Huitema LF, VanBergen M, Apschner A, Peterson-Maduro J, Logister I, Valentin G, Morelli LG, Oates AC, Schulte-Merker S.
|
Title |
Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock.
|
Journal |
Elife
|
Abstract |
Segmentation of the axial skeleton in amniotes depends on the segmentation clock, which patterns the paraxial mesoderm and the sclerotome. While the segmentation clock clearly operates in teleosts, the role of the sclerotome in establishing the axial skeleton is unclear. We severely disrupt zebrafish paraxial segmentation, yet observe a largely normal segmentation process of the chordacentra. We demonstrate that axial entpd5+ notochord sheath cells are responsible for chordacentrum mineralization, and serve as a marker for axial segmentation. While autonomous within the notochord sheath, entpd5 expression and centrum formation show some plasticity and can respond to myotome pattern. These observations reveal for the first time the dynamics of notochord segmentation in a teleost, and are consistent with an autonomous patterning mechanism that is influenced, but not determined by adjacent paraxial mesoderm. This behavior is not consistent with a clock-type mechanism in the notochord.
|
Volume |
7
|
Published |
2018-4-6
|
DOI |
10.7554/eLife.33843
|
PII |
33843
|
PMID |
29624170
|
PMC |
PMC5962341
|
MeSH |
Animals
Animals, Genetically Modified / embryology
Animals, Genetically Modified / genetics
Animals, Genetically Modified / physiology*
Biological Clocks*
Body Patterning*
Bone and Bones / embryology
Bone and Bones / physiology*
Embryo, Nonmammalian / cytology
Embryo, Nonmammalian / physiology
Gene Expression Regulation, Developmental
Mesoderm / embryology
Mesoderm / physiology
Mutation
Notochord / embryology
Notochord / physiology*
Pyrophosphatases / genetics
Pyrophosphatases / metabolism*
Zebrafish / embryology
Zebrafish / genetics
Zebrafish / physiology*
Zebrafish Proteins / genetics
Zebrafish Proteins / metabolism*
|
Resource |
Zebrafish |
SAGFF(LF)214A
USA:GFP |