論文 - 詳細
| RRC ID | 81355 |
|---|---|
| 著者 | Lleras Forero L, Narayanan R, Huitema LF, VanBergen M, Apschner A, Peterson-Maduro J, Logister I, Valentin G, Morelli LG, Oates AC, Schulte-Merker S. |
| タイトル | Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock. |
| ジャーナル | Elife |
| Abstract |
Segmentation of the axial skeleton in amniotes depends on the segmentation clock, which patterns the paraxial mesoderm and the sclerotome. While the segmentation clock clearly operates in teleosts, the role of the sclerotome in establishing the axial skeleton is unclear. We severely disrupt zebrafish paraxial segmentation, yet observe a largely normal segmentation process of the chordacentra. We demonstrate that axial entpd5+ notochord sheath cells are responsible for chordacentrum mineralization, and serve as a marker for axial segmentation. While autonomous within the notochord sheath, entpd5 expression and centrum formation show some plasticity and can respond to myotome pattern. These observations reveal for the first time the dynamics of notochord segmentation in a teleost, and are consistent with an autonomous patterning mechanism that is influenced, but not determined by adjacent paraxial mesoderm. This behavior is not consistent with a clock-type mechanism in the notochord. |
| 巻・号 | 7 |
| 公開日 | 2018-4-6 |
| DOI | 10.7554/eLife.33843 |
| PII | 33843 |
| PMID | 29624170 |
| PMC | PMC5962341 |
| MeSH | Animals Animals, Genetically Modified / embryology Animals, Genetically Modified / genetics Animals, Genetically Modified / physiology* Biological Clocks* Body Patterning* Bone and Bones / embryology Bone and Bones / physiology* Embryo, Nonmammalian / cytology Embryo, Nonmammalian / physiology Gene Expression Regulation, Developmental Mesoderm / embryology Mesoderm / physiology Mutation Notochord / embryology Notochord / physiology* Pyrophosphatases / genetics Pyrophosphatases / metabolism* Zebrafish / embryology Zebrafish / genetics Zebrafish / physiology* Zebrafish Proteins / genetics Zebrafish Proteins / metabolism* |
| IF | 7.08 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | SAGFF(LF)214A USA:GFP |