RRC ID 81434
Author Keyi Ye, Fengjiao Bu, Liyuan Zhong, Zhaonian Dong, Zhaoxu Ma, Zhanpeng Tang, Yu Zhang, Xueyong Yang, Xun Xu, Ertao Wang, William J. Lucas, Sanwen Huang, Huan Liu, Jianshu Zheng
Title Mapping the molecular landscape of Lotus japonicus nodule organogenesis through spatiotemporal transcriptomics
Journal Nature Communications
Abstract Legumes acquire nitrogen-fixing ability by forming root nodules. Transferring this capability to more crops could reduce our reliance on nitrogen fertilizers, thereby decreasing environmental pollution and agricultural production costs. Nodule organogenesis is complex, and a comprehensive transcriptomic atlas is crucial for understanding the underlying molecular events. Here, we utilized spatial transcriptomics to investigate the development of nodules in the model legume, Lotus japonicus. Our investigation has identified the developmental trajectories of two critical regions within the nodule: the infection zone and peripheral tissues. We reveal the underlying biological processes and provide gene sets to achieve symbiosis and material exchange, two essential aspects of nodulation. Among the candidate regulatory genes, we illustrate that LjNLP3, a transcription factor belonging to the NIN-LIKE PROTEIN family, orchestrates the transition of nodules from the differentiation to maturation. In summary, our research advances our understanding of nodule organogenesis and provides valuable data for developing symbiotic nitrogen-fixing crops.
Volume 15
Published 2024-7-29
DOI 10.1038/s41467-024-50737-8
PMID 39080318
PMC PMC11289483
MeSH Gene Expression Profiling Gene Expression Regulation, Plant* Lotus* / genetics Lotus* / growth & development Lotus* / metabolism Nitrogen Fixation* / genetics Organogenesis / genetics Organogenesis, Plant / genetics Plant Proteins* / genetics Plant Proteins* / metabolism Plant Root Nodulation / genetics Root Nodules, Plant* / genetics Root Nodules, Plant* / growth & development Root Nodules, Plant* / metabolism Root Nodules, Plant* / microbiology Spatio-Temporal Analysis Symbiosis / genetics Transcription Factors / genetics Transcription Factors / metabolism Transcriptome*
IF 12.121
Resource
Lotus / Glycine Gifu B-129