| RRC ID |
81500
|
| 著者 |
Ghosh S, Chakraborti S, Devi D, Sahu R, Mandal S, Mandal L.
|
| タイトル |
A conserved nutrient responsive axis mediates autophagic degradation of miRNA-mRNA hybrids in blood cell progenitors.
|
| ジャーナル |
Nucleic Acids Res
|
| Abstract |
In animals, microRNAs are amongst the primary non-coding RNAs involved in regulating the gene expression of a cell. Most mRNAs in a cell are targeted by one or many miRNAs. Although several mechanisms can be attributed to the degradation of miRNA and mRNA within a cell, but the involvement of autophagy in the clearance of miRNA and its target mRNA is not known. We discover a leucine-responsive axis in blood cell progenitors that can mediate an autophagy-directed degradation of miRNA-bound mRNA in Drosophila melanogaster and Homo sapiens. This previously unknown miRNA clearance axis is activated upon amino acid deprivation that can traffic miRNA-mRNA-loaded Argonaute for autophagic degradation in a p62-dependent manner. Thus, our research not only reports a novel axis that can address the turnover of a catalytically active miRISC but also elucidates a slicer-independent mechanism through which autophagy can selectively initiate the clearance of target mRNA.
|
| 巻・号 |
52(1)
|
| ページ |
385-403
|
| 公開日 |
2024-1-11
|
| DOI |
10.1093/nar/gkad1047
|
| PII |
7442544
|
| PMID |
37994707
|
| PMC |
PMC10783512
|
| MeSH |
Animals
Argonaute Proteins / genetics
Argonaute Proteins / metabolism
Autophagy / genetics
Blood Cells
Drosophila melanogaster / genetics
Drosophila melanogaster / metabolism
MicroRNAs* / genetics
MicroRNAs* / metabolism
RNA, Messenger / genetics
RNA, Messenger / metabolism
|
| IF |
11.502
|
| リソース情報 |
| ショウジョウバエ |
DGRC#105442 |
