| RRC ID |
81650
|
| Author |
Funakoshi T, Maeshima K, Yahata K, Sugano S, Imamoto F, Imamoto N.
|
| Title |
Two distinct human POM121 genes: requirement for the formation of nuclear pore complexes.
|
| Journal |
FEBS Lett
|
| Abstract |
Pom121 is one of the integral membrane components of the nuclear pore complex (NPC) in vertebrate cells. Unlike rodent cells carrying a single POM121 gene, human cells possess multiple POM121 gene loci on chromosome 7q11.23, as a consequence of complex segmental-duplications in this region during human evolution. In HeLa cells, two "full-length" Pom121 are transcribed and translated by two distinct genetic loci. RNAi experiments showed that efficient depletion of both Pom121 proteins significantly reduces assembled NPCs on nuclear envelope. Pom121-depletion also induced clustering of NPCs, indicating its role on maintenance of NPC structure/organization.
|
| Volume |
581(25)
|
| Pages |
4910-6
|
| Published |
2007-10-16
|
| DOI |
10.1016/j.febslet.2007.09.021
|
| PII |
S0014-5793(07)01003-4
|
| PMID |
17900573
|
| MeSH |
5' Flanking Region
Base Sequence
Cell Nucleus / ultrastructure
Chromosome Mapping
Cloning, Molecular
Exons
Gene Duplication*
HeLa Cells
Humans
Membrane Glycoproteins / chemistry
Membrane Glycoproteins / genetics*
Membrane Glycoproteins / metabolism
Molecular Sequence Data
Nuclear Pore / ultrastructure*
Protein Structure, Tertiary
RNA Interference
|
| Resource |
| DNA material |
pKS-POM121A-Venus (RDB20246)
pKS-POM121NC-Venus (RDB20247) |
