論文 - 詳細
| RRC ID | 81807 |
|---|---|
| 著者 | Inaba I, Hiramoto K, Yamate Y, Morita A, Tsutsumi T, Yasuda H, Sato EF. |
| タイトル | Inhibiting Neutrophil Extracellular Traps Protects against Ultraviolet B-Induced Skin Damage: Effects of Hochu-ekki-to and DNase I. |
| ジャーナル | Int J Mol Sci |
| Abstract |
UV-B radiation induces sunburn, and neutrophils are pivotal in this inflammation. In this study, we examined the potential involvement of neutrophil extracellular traps (NETs) in ultraviolet B (UVB)-induced skin inflammation, correlating the skin inflammation-mitigating effects of Hochu-ekki-to on UV-B irradiation and NETs. To elucidate NET distribution in the dorsal skin, male ICR mice, exposed to UVB irradiation, were immunohistologically analyzed to detect citrullinated histone H3 (citH3) and peptidylarginine deiminase 4 (PAD4). Reactive oxygen species (ROS) production in the bloodstream was analyzed. To establish the involvement of NET-released DNA in this inflammatory response, mice were UV-B irradiated following the intraperitoneal administration of DNase I. In vitro experiments were performed to scrutinize the impact of Hochu-ekki-to on A23187-induced NETs in neutrophil-like HL-60 cells. UV-B irradiation induced dorsal skin inflammation, coinciding with a significant increase in citH3 and PAD4 expression. Administration of DNase I attenuated UV-B-induced skin inflammation, whereas Hochu-ekki-to administration considerably suppressed the inflammation, correlating with diminished levels of citH3 and PAD4 in the dorsal skin. UV-B irradiation conspicuously augmented ROS and hydrogen peroxide (H2O2) production in the blood. Hochu-ekki-to significantly inhibited ROS and H2O2 generation. In vitro experiments demonstrated that Hochu-ekki-to notably inhibited A23187-induced NETs in differentiated neutrophil-like cells. Hence, NETs have been implicated in UV-B-induced skin inflammation, and their inhibition reduces cutaneous inflammation. Additionally, Hochu-ekki-to mitigated skin inflammation by impeding neutrophil infiltration and NETs in the dorsal skin of mice. |
| 巻・号 | 25(3) |
| 公開日 | 2024-1-31 |
| DOI | 10.3390/ijms25031723 |
| PII | ijms25031723 |
| PMID | 38339001 |
| PMC | PMC10855064 |
| MeSH | Animals Calcimycin / pharmacology Deoxyribonuclease I* / metabolism Deoxyribonuclease I* / pharmacology Drugs, Chinese Herbal* Extracellular Traps* / drug effects Extracellular Traps* / radiation effects Histones / metabolism Hydrogen Peroxide / metabolism Inflammation / metabolism Male Mice Mice, Inbred ICR Neutrophils / metabolism Protein-Arginine Deiminases / metabolism Reactive Oxygen Species / metabolism Ultraviolet Rays* / adverse effects |
| IF | 4.556 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | News |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 7.0 |
| リソース情報 | |
| ヒト・動物細胞 | HL-60(RCB3683) |