Reference - Detail
| RRC ID | 81940 |
|---|---|
| Author | Lyu XH, Yang YS, Pan ZQ, Ning SK, Suo F, Du LL. |
| Title | An improved tetracycline-inducible expression system for fission yeast. |
| Journal | J Cell Sci |
| Abstract |
The ability to manipulate gene expression is valuable for elucidating gene function. In the fission yeast Schizosaccharomyces pombe, the most widely used regulatable expression system is the nmt1 promoter and its two attenuated variants. However, these promoters have limitations, including a long lag, incompatibility with rich media and unsuitability for non-dividing cells. Here, we present a tetracycline-inducible system free of these shortcomings. Our system features the enotetS promoter, which achieves a similar induced level and a higher induction ratio compared to the nmt1 promoter, without exhibiting a lag. Additionally, our system includes four weakened enotetS variants, offering an expression range similar to that of the nmt1 series promoters but with more intermediate levels. To enhance usability, each promoter is combined with a Tet-repressor-expressing cassette in an integration plasmid. Importantly, our system can be used in non-dividing cells, enabling the development of a synchronous meiosis induction method with high spore viability. Moreover, our system allows for the shutdown of gene expression and the generation of conditional loss-of-function mutants. This system provides a versatile and powerful tool for manipulating gene expression in fission yeast. |
| Volume | 137(21) |
| Published | 2024-11-1 |
| DOI | 10.1242/jcs.263404 |
| PII | 362156 |
| PMID | 39318285 |
| MeSH | Gene Expression Regulation, Fungal* / drug effects Meiosis / drug effects Meiosis / genetics Plasmids / genetics Plasmids / metabolism Promoter Regions, Genetic* / genetics Schizosaccharomyces* / drug effects Schizosaccharomyces* / genetics Schizosaccharomyces* / metabolism Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism Tetracycline* / pharmacology |
| IF | 4.573 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 4 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Yeast | FYP6242–FYP6246 |