RRC ID 82046
Author Howard L, Ishikawa Y, Katayama T, Park SJ, Hill MJ, Blake DJ, Nishida K, Hayashi R, Quantock AJ.
Title Single-cell transcriptomics reveals the molecular basis of human iPS cell differentiation into ectodermal ocular lineages.
Journal Commun Biol
Abstract The generation of a self-formed, ectodermal, autonomous multi-zone (SEAM) from human induced pluripotent stem cells (hiPSCs) offers a unique perspective to study the dynamics of ocular cell differentiation over time. Here, by utilising single-cell transcriptomics, we have (i) identified, (ii) molecularly characterised and (iii) ascertained the developmental trajectories of ectodermally-derived ocular cell populations which emerge within SEAMs as they form. Our analysis reveals interdependency between tissues of the early eye and delineates the sequential formation and maturation of distinct cell types over a 12-week period. We demonstrate a progression from pluripotency through to tissue specification and differentiation which encompasses both surface ectodermal and neuroectodermal ocular lineages and the generation of iPSC-derived components of the developing cornea, conjunctiva, lens, and retina. Our findings not only advance the understanding of ocular development in a stem cell-based system of human origin, but also establish a robust methodological paradigm for exploring cellular and molecular dynamics during SEAM formation at single-cell resolution and highlight the potential of hiPSC-derived systems as powerful platforms for modelling human eye development and disease.
Volume 7(1)
Pages 1495
Published 2024-11-12
DOI 10.1038/s42003-024-07130-4
PII 10.1038/s42003-024-07130-4
PMID 39532995
PMC PMC11557866
MeSH Cell Differentiation* / genetics Cell Lineage / genetics Ectoderm* / cytology Ectoderm* / metabolism Eye / cytology Eye / growth & development Gene Expression Profiling Humans Induced Pluripotent Stem Cells* / cytology Induced Pluripotent Stem Cells* / metabolism Single-Cell Analysis* / methods Transcriptome*
Resource
Human and Animal Cells 201B7(HPS0063)