| RRC ID |
82046
|
| 著者 |
Howard L, Ishikawa Y, Katayama T, Park SJ, Hill MJ, Blake DJ, Nishida K, Hayashi R, Quantock AJ.
|
| タイトル |
Single-cell transcriptomics reveals the molecular basis of human iPS cell differentiation into ectodermal ocular lineages.
|
| ジャーナル |
Commun Biol
|
| Abstract |
The generation of a self-formed, ectodermal, autonomous multi-zone (SEAM) from human induced pluripotent stem cells (hiPSCs) offers a unique perspective to study the dynamics of ocular cell differentiation over time. Here, by utilising single-cell transcriptomics, we have (i) identified, (ii) molecularly characterised and (iii) ascertained the developmental trajectories of ectodermally-derived ocular cell populations which emerge within SEAMs as they form. Our analysis reveals interdependency between tissues of the early eye and delineates the sequential formation and maturation of distinct cell types over a 12-week period. We demonstrate a progression from pluripotency through to tissue specification and differentiation which encompasses both surface ectodermal and neuroectodermal ocular lineages and the generation of iPSC-derived components of the developing cornea, conjunctiva, lens, and retina. Our findings not only advance the understanding of ocular development in a stem cell-based system of human origin, but also establish a robust methodological paradigm for exploring cellular and molecular dynamics during SEAM formation at single-cell resolution and highlight the potential of hiPSC-derived systems as powerful platforms for modelling human eye development and disease.
|
| 巻・号 |
7(1)
|
| ページ |
1495
|
| 公開日 |
2024-11-12
|
| DOI |
10.1038/s42003-024-07130-4
|
| PII |
10.1038/s42003-024-07130-4
|
| PMID |
39532995
|
| PMC |
PMC11557866
|
| MeSH |
Cell Differentiation* / genetics
Cell Lineage / genetics
Ectoderm* / cytology
Ectoderm* / metabolism
Eye / cytology
Eye / growth & development
Gene Expression Profiling
Humans
Induced Pluripotent Stem Cells* / cytology
Induced Pluripotent Stem Cells* / metabolism
Single-Cell Analysis* / methods
Transcriptome*
|
| IF |
4.165
|
| リソース情報 |
| ヒト・動物細胞 |
201B7(HPS0063) |
