| RRC ID |
82102
|
| 著者 |
Hosokawa K, Ishimaru H, Watanabe T, Fujimuro M.
|
| タイトル |
Pax5 mediates the transcriptional activation of the CD81 gene.
|
| ジャーナル |
Sci Rep
|
| Abstract |
CD81 is an integral membrane protein of the tetraspanin family and forms complexes with a variety of other cell surface membrane proteins. CD81 is involved in cell migration and B cell activation. However, the mechanism of the transcriptional regulation of the CD81 gene remains unclear. Here, we revealed that CD81 transcriptional activation was required for binding of the transcription factor Pax5 at the Pax5-binding sequence (-54)GCGGGAC(-48) located upstream of the transcriptional start site (TSS) of the CD81 gene. The reporter assay showed that the DNA sequence between - 130 and - 39 bp upstream of the TSS of the CD81 gene had promoter activity for CD81 transcription. The DNA sequence between - 130 and - 39 bp upstream of TSS of CD81 harbors two potential Pax5-binding sequences (-87)GCGTGAG(-81) and (-54)GCGGGAC(-48). Reporter, electrophoresis mobility shift, and chromatin immunoprecipitation (ChIP) assays disclosed that Pax5 bound to the (-54)GCGGGAC(-48) in the promoter region of the CD81 gene in order to activate CD81 transcription. Pax5 overexpression increased the expression level of CD81 protein, while the Pax5-knockdown by shRNA decreased CD81 expression. Moreover, we found that the expression level of CD81 was positively correlated with Pax5 expression in human tumor cell lines. Because CD81 was reported to be involved in cell migration, we evaluated the effects of Pax5 overexpression by wound healing and transwell assays. The data showed that overexpression of either Pax5 or CD81 promoted the epithelial cell migration. Thus, our findings provide insights into the transcriptional mechanism of the CD81 gene through transcription factor Pax5.
|
| 巻・号 |
11(1)
|
| ページ |
22919
|
| 公開日 |
2021-11-25
|
| DOI |
10.1038/s41598-021-02082-9
|
| PII |
10.1038/s41598-021-02082-9
|
| PMID |
34824296
|
| PMC |
PMC8616915
|
| MeSH |
A549 Cells
Binding Sites
Cell Movement
Gene Expression Regulation, Neoplastic
HEK293 Cells
HeLa Cells
Humans
MCF-7 Cells
Neoplasm Invasiveness
Neoplasms / genetics
Neoplasms / metabolism*
Neoplasms / pathology
PAX5 Transcription Factor / genetics
PAX5 Transcription Factor / metabolism*
Promoter Regions, Genetic*
Protein Binding
Tetraspanin 28 / genetics
Tetraspanin 28 / metabolism*
Transcriptional Activation*
|
| IF |
3.998
|
| リソース情報 |
| ヒト・動物細胞 |
HeLa(RCB0007)
293T(RCB2202) |
