論文 - 詳細
| RRC ID | 82154 |
|---|---|
| 著者 | Kanadome T, Hoshino N, Nagai T, Yagi T, Matsuda T. |
| タイトル | Protocol to visualize trans-interaction of clustered protocadherin using cIPAD, a fluorescent indicator, in cultured human cells and mouse neurons. |
| ジャーナル | STAR Protoc |
| Abstract |
cIPAD is a fluorescent indicator that allows the visualization of trans-interactions of clustered protocadherin (Pcdh), a cell adhesion molecule that mediates neuronal self-recognition. We describe steps for using HEK293T cells to visualize Pcdh trans-interactions across cells as a preliminary experiment before using dissociated mouse neurons. We then detail procedures for visualizing Pcdh trans-interactions between processes originating from the same neurons, which are considered as Pcdh-mediated neuronal self-recognition. For complete details on the use and execution of this protocol, please refer to Kanadome et al.1. |
| 巻・号 | 5(1) |
| ページ | 102844 |
| 公開日 | 2024-3-15 |
| DOI | 10.1016/j.xpro.2024.102844 |
| PII | S2666-1667(24)00009-1 |
| PMID | 38277267 |
| PMC | PMC10840325 |
| MeSH | Animals Cadherins* / genetics Cadherins* / metabolism Cell Adhesion HEK293 Cells Humans Mice Neurons / metabolism Protocadherins* |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 293T(RCB2202) |