| RRC ID |
82154
|
| Author |
Kanadome T, Hoshino N, Nagai T, Yagi T, Matsuda T.
|
| Title |
Protocol to visualize trans-interaction of clustered protocadherin using cIPAD, a fluorescent indicator, in cultured human cells and mouse neurons.
|
| Journal |
STAR Protoc
|
| Abstract |
cIPAD is a fluorescent indicator that allows the visualization of trans-interactions of clustered protocadherin (Pcdh), a cell adhesion molecule that mediates neuronal self-recognition. We describe steps for using HEK293T cells to visualize Pcdh trans-interactions across cells as a preliminary experiment before using dissociated mouse neurons. We then detail procedures for visualizing Pcdh trans-interactions between processes originating from the same neurons, which are considered as Pcdh-mediated neuronal self-recognition. For complete details on the use and execution of this protocol, please refer to Kanadome et al.1.
|
| Volume |
5(1)
|
| Pages |
102844
|
| Published |
2024-3-15
|
| DOI |
10.1016/j.xpro.2024.102844
|
| PII |
S2666-1667(24)00009-1
|
| PMID |
38277267
|
| PMC |
PMC10840325
|
| MeSH |
Animals
Cadherins* / genetics
Cadherins* / metabolism
Cell Adhesion
HEK293 Cells
Humans
Mice
Neurons / metabolism
Protocadherins*
|
| Resource |
| Human and Animal Cells |
293T(RCB2202) |
