| RRC ID |
82158
|
| Author |
Springall L, Hughes CD, Simons M, Azinas S, Van Houten B, Kad NM.
|
| Title |
Recruitment of UvrBC complexes to UV-induced damage in the absence of UvrA increases cell survival.
|
| Journal |
Nucleic Acids Res
|
| Abstract |
Nucleotide excision repair (NER) is the primary mechanism for removal of ultraviolet light (UV)-induced DNA photoproducts and is mechanistically conserved across all kingdoms of life. Bacterial NER involves damage recognition by UvrA2 and UvrB, followed by UvrC-mediated incision either side of the lesion. Here, using a combination of in vitro and in vivo single-molecule studies we show that a UvrBC complex is capable of lesion identification in the absence of UvrA. Single-molecule analysis of eGFP-labelled UvrB and UvrC in living cells showed that UV damage caused these proteins to switch from cytoplasmic diffusion to stable complexes on DNA. Surprisingly, ectopic expression of UvrC in a uvrA deleted strain increased UV survival. These data provide evidence for a previously unrealized mechanism of survival that can occur through direct lesion recognition by a UvrBC complex.
|
| Volume |
46(3)
|
| Pages |
1256-1265
|
| Published |
2018-2-16
|
| DOI |
10.1093/nar/gkx1244
|
| PII |
4731550
|
| PMID |
29240933
|
| PMC |
PMC5814901
|
| MeSH |
Adenosine Triphosphatases / deficiency
Adenosine Triphosphatases / genetics*
Bacillus / chemistry
Bacillus / genetics
Bacillus / metabolism
DNA Damage
DNA Helicases / genetics*
DNA Helicases / metabolism
DNA Repair*
DNA, Bacterial / genetics*
DNA, Bacterial / metabolism
DNA-Binding Proteins / deficiency
DNA-Binding Proteins / genetics*
Endodeoxyribonucleases / genetics*
Endodeoxyribonucleases / metabolism
Escherichia coli / genetics
Escherichia coli / metabolism
Escherichia coli / radiation effects*
Escherichia coli Proteins / genetics*
Escherichia coli Proteins / metabolism
Gene Deletion
Gene Expression Regulation, Bacterial
Genes, Reporter
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
Microbial Viability / genetics
Microbial Viability / radiation effects
Protein Binding
Single Molecule Imaging / methods
Ultraviolet Rays
|
| Resource |
| Prokaryotes E. coli |
|
