Reference - Detail
| RRC ID | 82158 |
|---|---|
| Author | Springall L, Hughes CD, Simons M, Azinas S, Van Houten B, Kad NM. |
| Title | Recruitment of UvrBC complexes to UV-induced damage in the absence of UvrA increases cell survival. |
| Journal | Nucleic Acids Res |
| Abstract |
Nucleotide excision repair (NER) is the primary mechanism for removal of ultraviolet light (UV)-induced DNA photoproducts and is mechanistically conserved across all kingdoms of life. Bacterial NER involves damage recognition by UvrA2 and UvrB, followed by UvrC-mediated incision either side of the lesion. Here, using a combination of in vitro and in vivo single-molecule studies we show that a UvrBC complex is capable of lesion identification in the absence of UvrA. Single-molecule analysis of eGFP-labelled UvrB and UvrC in living cells showed that UV damage caused these proteins to switch from cytoplasmic diffusion to stable complexes on DNA. Surprisingly, ectopic expression of UvrC in a uvrA deleted strain increased UV survival. These data provide evidence for a previously unrealized mechanism of survival that can occur through direct lesion recognition by a UvrBC complex. |
| Volume | 46(3) |
| Pages | 1256-1265 |
| Published | 2018-2-16 |
| DOI | 10.1093/nar/gkx1244 |
| PII | 4731550 |
| PMID | 29240933 |
| PMC | PMC5814901 |
| MeSH | Adenosine Triphosphatases / deficiency Adenosine Triphosphatases / genetics* Bacillus / chemistry Bacillus / genetics Bacillus / metabolism DNA Damage DNA Helicases / genetics* DNA Helicases / metabolism DNA Repair* DNA, Bacterial / genetics* DNA, Bacterial / metabolism DNA-Binding Proteins / deficiency DNA-Binding Proteins / genetics* Endodeoxyribonucleases / genetics* Endodeoxyribonucleases / metabolism Escherichia coli / genetics Escherichia coli / metabolism Escherichia coli / radiation effects* Escherichia coli Proteins / genetics* Escherichia coli Proteins / metabolism Gene Deletion Gene Expression Regulation, Bacterial Genes, Reporter Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Microbial Viability / genetics Microbial Viability / radiation effects Protein Binding Single Molecule Imaging / methods Ultraviolet Rays |
| Altmetric score |
オルトメトリクス指標項目
|
| Total number of mentions | 0 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Prokaryotes E. coli | |