RRC ID 82616
Author Qi X, Wu Z, Li J, Mo X, Wu S, Chu J, Wu P.
Title AtCYT-INV1, a neutral invertase, is involved in osmotic stress-induced inhibition on lateral root growth in Arabidopsis.
Journal Plant Mol Biol
Abstract Neutral/Alkaline invertases are unique to plant and photosynthetic bacteria. The function of Neutral/Alkaline invertases in plant development is not clear so far. In this study, we isolated an Arabidopsis (Col-0) mutant insensitive to osmotic stress-induced inhibition on lateral root growth. Map-based cloning reveals that a neutral invertase gene (AtCYT-INV1) was point-mutated. The mutant Atcyt-inv1 showed short primary root, smaller size of leaves and siliques, and promotion of the reproductive compared to the wild type (WT). Carbohydrate measurement showed that sucrose is accumulated and glucose is reduced in the mutant Atcyt-inv1 under normal and 3% mannitol treatments. Taken together, AtCYT-INV1 plays multiple roles in plant development and is involved in osmotic stress-induced inhibition on lateral root growth by controlling the concentration of hexose in cells.
Volume 64(5)
Pages 575-87
Published 2007-7-1
DOI 10.1007/s11103-007-9177-4
PMID 17508130
MeSH Abscisic Acid / pharmacology Amino Acid Sequence Arabidopsis / enzymology* Arabidopsis / genetics Arabidopsis / growth & development Arabidopsis Proteins / chemistry Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Cloning, Molecular Conserved Sequence DNA Primers Fructose / metabolism Genetic Vectors Glucose / metabolism Kinetics Mannitol / metabolism Molecular Sequence Data Mutagenesis Phylogeny Plant Roots / growth & development* Recombinant Proteins / metabolism Reverse Transcriptase Polymerase Chain Reaction Sequence Alignment Sequence Homology, Amino Acid beta-Fructofuranosidase / chemistry beta-Fructofuranosidase / genetics beta-Fructofuranosidase / metabolism*
IF 3.302
Resource
Arabidopsis / Cultured plant cells, genes pda06259