RRC ID 82680
Author Xiao J, Khan MM, Vemula S, Tian J, LeDoux MS.
Title Consequences of Cre-mediated deletion of Ciz1 exon 5 in mice.
Journal FEBS Lett
Abstract CIZ1 plays a role in DNA synthesis at the G1/S checkpoint. Ciz1 gene-trap null mice manifest motor dysfunction, cell-cycle abnormalities, and DNA damage. In contrast, it has previously been reported that mouse embryonic fibroblasts derived from presumed Ciz1 knock-out mice (Ciz1tm1.1Homy/tm1.1Homy ) generated by crossing Cre-expressing mice with exon 5-floxed mice (Ciz1tm1Homy/tm1Homy ) do not exhibit evidence of enhanced DNA damage following γ-irradiation or cell-cycle defects. Here, we report that Ciz1tm1.1Homy/tm1.1Homy mice show loss of Ciz1 exon 5 but are neurologically normal and express abnormal transcripts (Ciz1ΔE5/ΔE5 mice) that are translated into one or more proteins of approximate wild-type size. Therefore, Ciz1tm1.1Homy/tm1.1Homy mice (Ciz1ΔE5/ΔE5 ) lose residues encoded by exon 5 but may gain function from novel amino acid sequences.
Volume 592(18)
Pages 3101-3110
Published 2018-9-1
DOI 10.1002/1873-3468.13221
PMID 30098009
PMC PMC6275157
MeSH Animals Cell Cycle / genetics* Cells, Cultured DNA Damage* Embryo, Mammalian / cytology Exons / genetics* Female Fibroblasts / cytology Fibroblasts / metabolism Integrases / genetics Integrases / metabolism Male Mice, Inbred C57BL Mice, Knockout Mice, Transgenic Motor Activity / genetics Nuclear Proteins / genetics* Nuclear Proteins / metabolism
Resource
Mice RBRC05198