| RRC ID |
82680
|
| 著者 |
Xiao J, Khan MM, Vemula S, Tian J, LeDoux MS.
|
| タイトル |
Consequences of Cre-mediated deletion of Ciz1 exon 5 in mice.
|
| ジャーナル |
FEBS Lett
|
| Abstract |
CIZ1 plays a role in DNA synthesis at the G1/S checkpoint. Ciz1 gene-trap null mice manifest motor dysfunction, cell-cycle abnormalities, and DNA damage. In contrast, it has previously been reported that mouse embryonic fibroblasts derived from presumed Ciz1 knock-out mice (Ciz1tm1.1Homy/tm1.1Homy ) generated by crossing Cre-expressing mice with exon 5-floxed mice (Ciz1tm1Homy/tm1Homy ) do not exhibit evidence of enhanced DNA damage following γ-irradiation or cell-cycle defects. Here, we report that Ciz1tm1.1Homy/tm1.1Homy mice show loss of Ciz1 exon 5 but are neurologically normal and express abnormal transcripts (Ciz1ΔE5/ΔE5 mice) that are translated into one or more proteins of approximate wild-type size. Therefore, Ciz1tm1.1Homy/tm1.1Homy mice (Ciz1ΔE5/ΔE5 ) lose residues encoded by exon 5 but may gain function from novel amino acid sequences.
|
| 巻・号 |
592(18)
|
| ページ |
3101-3110
|
| 公開日 |
2018-9-1
|
| DOI |
10.1002/1873-3468.13221
|
| PMID |
30098009
|
| PMC |
PMC6275157
|
| MeSH |
Animals
Cell Cycle / genetics*
Cells, Cultured
DNA Damage*
Embryo, Mammalian / cytology
Exons / genetics*
Female
Fibroblasts / cytology
Fibroblasts / metabolism
Integrases / genetics
Integrases / metabolism
Male
Mice, Inbred C57BL
Mice, Knockout
Mice, Transgenic
Motor Activity / genetics
Nuclear Proteins / genetics*
Nuclear Proteins / metabolism
|
| IF |
3.057
|
| リソース情報 |
| 実験動物マウス |
RBRC05198 |
