論文 - 詳細
| RRC ID | 82680 |
|---|---|
| 著者 | Xiao J, Khan MM, Vemula S, Tian J, LeDoux MS. |
| タイトル | Consequences of Cre-mediated deletion of Ciz1 exon 5 in mice. |
| ジャーナル | FEBS Lett |
| Abstract |
CIZ1 plays a role in DNA synthesis at the G1/S checkpoint. Ciz1 gene-trap null mice manifest motor dysfunction, cell-cycle abnormalities, and DNA damage. In contrast, it has previously been reported that mouse embryonic fibroblasts derived from presumed Ciz1 knock-out mice (Ciz1tm1.1Homy/tm1.1Homy ) generated by crossing Cre-expressing mice with exon 5-floxed mice (Ciz1tm1Homy/tm1Homy ) do not exhibit evidence of enhanced DNA damage following γ-irradiation or cell-cycle defects. Here, we report that Ciz1tm1.1Homy/tm1.1Homy mice show loss of Ciz1 exon 5 but are neurologically normal and express abnormal transcripts (Ciz1ΔE5/ΔE5 mice) that are translated into one or more proteins of approximate wild-type size. Therefore, Ciz1tm1.1Homy/tm1.1Homy mice (Ciz1ΔE5/ΔE5 ) lose residues encoded by exon 5 but may gain function from novel amino acid sequences. |
| 巻・号 | 592(18) |
| ページ | 3101-3110 |
| 公開日 | 2018-9-1 |
| DOI | 10.1002/1873-3468.13221 |
| PMID | 30098009 |
| PMC | PMC6275157 |
| MeSH | Animals Cell Cycle / genetics* Cells, Cultured DNA Damage* Embryo, Mammalian / cytology Exons / genetics* Female Fibroblasts / cytology Fibroblasts / metabolism Integrases / genetics Integrases / metabolism Male Mice, Inbred C57BL Mice, Knockout Mice, Transgenic Motor Activity / genetics Nuclear Proteins / genetics* Nuclear Proteins / metabolism |
| IF | 3.057 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 実験動物マウス | RBRC05198 |