Reference - Detail
| RRC ID | 82804 |
|---|---|
| Author | Tao S, Witte M, Bryson-Richardson RJ, Currie PD, Hogan BM, Schulte-Merker S. |
| Title | Zebrafish prox1b mutants develop a lymphatic vasculature, and prox1b does not specifically mark lymphatic endothelial cells. |
| Journal | PLoS One |
| Abstract |
BACKGROUND:The expression of the Prospero homeodomain transcription factor (Prox1) in a subset of cardinal venous cells specifies the lymphatic lineage in mice. Prox1 is also indispensible for the maintenance of lymphatic cell fate, and is therefore considered a master control gene for lymphangiogenesis in mammals. In zebrafish, there are two prox1 paralogues, the previously described prox1 (also known as prox1a) and the newly identified prox1b. PRINCIPAL FINDINGS:To investigate the role of the prox1b gene in zebrafish lymphangiogenesis, we knocked-down prox1b and found that depletion of prox1b mRNA did not cause lymphatic defects. We also generated two different prox1b mutant alleles, and maternal-zygotic homozygous mutant embryos were viable and did not show any lymphatic defects. Furthermore, the expression of prox1b was not restricted to lymphatic vessels during zebrafish development. CONCLUSION:We conclude that Prox1b activity is not essential for embryonic lymphatic development in zebrafish. |
| Volume | 6(12) |
| Pages | e28934 |
| Published | 2011-1-1 |
| DOI | 10.1371/journal.pone.0028934 |
| PII | PONE-D-11-15289 |
| PMID | 22216143 |
| PMC | PMC3247213 |
| MeSH | Animals Homeodomain Proteins / genetics* Lymphangiogenesis Lymphatic Vessels / cytology* Mutation* Prospero-Related Homeobox 1 Protein Tumor Suppressor Proteins / genetics* Zebrafish / embryology Zebrafish / genetics* |
| IF | 2.74 |
| Resource | |
| Zebrafish | SAGFF(LF)27C UAS:GFP |