論文 - 詳細
| RRC ID | 83066 |
|---|---|
| 著者 | Nomura W, Inoue Y. |
| タイトル | Activation of the cell wall integrity pathway negatively regulates TORC2-Ypk1/2 signaling through blocking eisosome disassembly in Saccharomyces cerevisiae. |
| ジャーナル | Commun Biol |
| Abstract |
The target of rapamycin complex 2 (TORC2) signaling is associated with plasma membrane (PM) integrity. In Saccharomyces cerevisiae, TORC2-Ypk1/2 signaling controls sphingolipid biosynthesis, and Ypk1/2 phosphorylation by TORC2 under PM stress conditions is increased in a Slm1/2-dependent manner, under which Slm1 is known to be released from an eisosome, a furrow-like invagination PM structure. However, it remains unsolved how the activation machinery of TORC2-Ypk1/2 signaling is regulated. Here we show that edelfosine, a synthetic lysophospholipid analog, inhibits the activation of TORC2-Ypk1/2 signaling, and the cell wall integrity (CWI) pathway is involved in this inhibitory effect. The activation of CWI pathway blocked the eisosome disassembly promoted by PM stress and the release of Slm1 from eisosomes. Constitutive activation of TORC2-Ypk1/2 signaling exhibited increased sensitivity to cell wall stress. We propose that the CWI pathway negatively regulates the TORC2-Ypk1/2 signaling, which is involved in the regulatory mechanism to ensure the proper stress response to cell wall damage. |
| 巻・号 | 7(1) |
| ページ | 722 |
| 公開日 | 2024-6-11 |
| DOI | 10.1038/s42003-024-06411-2 |
| PII | 10.1038/s42003-024-06411-2 |
| PMID | 38862688 |
| PMC | PMC11166964 |
| MeSH | Cell Wall* / drug effects Cell Wall* / metabolism Mechanistic Target of Rapamycin Complex 2* / genetics Mechanistic Target of Rapamycin Complex 2* / metabolism Phosphorylation Protein Kinases Protein Serine-Threonine Kinases Saccharomyces cerevisiae* / drug effects Saccharomyces cerevisiae* / genetics Saccharomyces cerevisiae* / metabolism Saccharomyces cerevisiae Proteins* / genetics Saccharomyces cerevisiae Proteins* / metabolism Signal Transduction* rab GTP-Binding Proteins / genetics rab GTP-Binding Proteins / metabolism |
| IF | 4.165 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | BY23849 |