Reference - Detail
| RRC ID | 83115 |
|---|---|
| Author | Sharmin T, Morshed S, Ushimaru T. |
| Title | PP2A promotes ESCRT-0 complex formation on vacuolar membranes and microautophagy induction after TORC1 inactivation. |
| Journal | Biochem Biophys Res Commun |
| Abstract |
Deformation of vacuolar membranes mediated by endosomal sorting complex required for transport (ESCRT) is necessary for microautophagy. Target of rapamycin complex 1 (TORC1) protein kinase negatively regulates ESCRT-0 (Vps27-Hse1) recruitment onto vacuolar membranes and microautophagy induction. However, whether and how protein phosphatase regulates these events is unknown. Here, we show that the TORC1-downstream protein phosphatase PP2A-Cdc55 is important for these events after TORC1 inactivation in budding yeast. Loss of PP2A-Cdc55 compromised vacuolar localization of Hse1, but not Vps27. This study revealed that the orchestrated action of PP2A induces microautophagy upon TORC1 inactivation. |
| Volume | 524(3) |
| Pages | 614-620 |
| Published | 2020-4-9 |
| DOI | 10.1016/j.bbrc.2020.01.129 |
| PII | S0006-291X(20)30212-6 |
| PMID | 32029270 |
| MeSH | Cell Cycle Proteins / metabolism Endosomal Sorting Complexes Required for Transport / metabolism* Intracellular Membranes / metabolism* Mechanistic Target of Rapamycin Complex 1 / metabolism* Microautophagy* Protein Binding Protein Phosphatase 2 / metabolism Receptors, Cytoplasmic and Nuclear / metabolism Saccharomyces cerevisiae / cytology* Saccharomyces cerevisiae / metabolism* Saccharomyces cerevisiae Proteins / metabolism* Vacuoles / metabolism* |
| IF | 2.985 |
| Resource | |
| Yeast | |