RRC ID 83354
著者 Itaya M, Fujita K, Kuroki A, Tsuge K.
タイトル Bottom-up genome assembly using the Bacillus subtilis genome vector.
ジャーナル Nat Methods
Abstract We established a protocol to construct complete recombinant genomes from their small contiguous DNA pieces and obtained the genomes of mouse mitochondrion and rice chloroplast using a B. subtilis genome (BGM) vector. This method allows the design of any recombinant genomes, valuable not only for fundamental research in systems biology and synthetic biology but also for various applications in the life sciences.
巻・号 5(1)
ページ 41-3
公開日 2008-1-1
DOI 10.1038/nmeth1143
PII nmeth1143
PMID 18066072
MeSH Bacillus subtilis / genetics* Bacterial Proteins / genetics* DNA, Bacterial / genetics* Genetic Engineering / methods* Genetic Vectors / genetics* Genome, Bacterial / genetics* Recombinant Proteins / genetics*
IF 30.822
オルトメトリクス指標
オルトメトリクス指標項目
最多言及媒体 Patent(IFI CLAIMS)
各媒体での言及数の合計 16
過去6か月間でのオルトメトリクス指標の変動値 0.0
リソース情報
原核生物(枯草菌) MBP34 MBP35