論文 - 詳細
| RRC ID | 83498 |
|---|---|
| 著者 | Morikawa K, Nishida H, Imami K, Ishihama Y. |
| タイトル | One-step N-Terminomics Based on Isolation of Protein N-Terminal Peptides From LysargiNase Digests by Tip-Based Strong Cation Exchange Chromatography. |
| ジャーナル | Mol Cell Proteomics |
| Abstract |
We have developed a one-step isolation method for protein N-terminal peptides from LysargiNase digests by pipette tip-based strong cation exchange (SCX) chromatography. This CHAMP-N (CHromatographic AMplification of Protein N-terminal peptides) method using disposable and parallel-processable SCX tips instead of conventional HPLC SCX columns facilitates simple, sensitive, reproducible, and high-throughput N-terminomic profiling without sacrificing the high identification numbers and selectivity achieved by the HPLC-based method. By applying the CHAMP-N method to HEK293T cells, we identified novel cleavage sites for signal and transit peptides and non-canonical translation initiation sites. Finally, for proteome-wide terminomics, we present a simple and comprehensive N- and C-terminomics platform employing three different tip-based approaches, including CHAMP-N, in which protease digestion and one-step isolation by tip LC are commonly used to achieve complementary terminome coverages. |
| 巻・号 | 23(9) |
| ページ | 100820 |
| 公開日 | 2024-9-1 |
| DOI | 10.1016/j.mcpro.2024.100820 |
| PII | S1535-9476(24)00110-5 |
| PMID | 39069075 |
| PMC | PMC11382313 |
| MeSH | Chromatography, Ion Exchange / methods HEK293 Cells Humans Peptides* / chemistry Peptides* / metabolism Proteome / metabolism Proteomics* / methods |
| IF | 4.87 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 293T(RCB2202) |