Reference - Detail
| RRC ID | 83688 |
|---|---|
| Author | Zhang R, Danshiitsoodol N, Noda M, Yonezawa S, Kanno K, Sugiyama M. |
| Title | Stevia Leaf Extract Fermented with Plant-Derived Lactobacillus plantarum SN13T Displays Anticancer Activity to Pancreatic Cancer PANC-1 Cell Line. |
| Journal | Int J Mol Sci |
| Abstract |
Pancreatic cancer is a highly malignant tumor that remains a significant global health burden. In this study, we demonstrated the anticancer potential of stevia leaf extract fermented with plant-derived Lactobacillus (L.) plantarum SN13T strain. Evaluation of antioxidant capacity (including DPPH and ABTS radical scavenging activities and H2O2-induced oxidative damage repair in HEK-293 cells), as well as cytotoxicity against pancreatic cancer cells (PANC-1) and non-cancerous human embryonic kidney (HEK-293), revealed that the fermented extract exhibited significantly enhanced antioxidant activity and cytotoxicity against PANC-1 cells while showing minimal toxicity to HEK-293 cells compared to the unfermented extract. Further, validation through clonogenic, migration, and wound-healing assays demonstrated that the fermented extract effectively inhibited the proliferation and migration of PANC-1 cells. The active compound in the fermented extract has been identified as chlorogenic acid methyl ester (CAME), with a concentration of 374.4 μg/mL. Flow cytometry analysis indicated that CAME significantly arrested PANC-1 cells in the G0/G1 phase and induced apoptosis. Furthermore, CAME upregulated the expression of pro-apoptotic genes Bax, Bad, Caspase-3/9, Cytochrome c, and E-cadherin, while downregulating the anti-apoptotic gene Bcl-2. These findings suggest that CAME exerts potent cytotoxic effects on PANC-1 cells by inhibiting cell proliferation and migration, arresting the cell cycle, and regulating apoptosis-related gene expression. In conclusion, stevia leaf extract fermented with L. plantarum SN13T, which contains CAME, may serve as a promising candidate for pancreatic cancer treatment. |
| Volume | 26(9) |
| Published | 2025-4-28 |
| DOI | 10.3390/ijms26094186 |
| PII | ijms26094186 |
| PMID | 40362423 |
| PMC | PMC12071683 |
| MeSH | Antineoplastic Agents, Phytogenic* / pharmacology Antioxidants / pharmacology Apoptosis / drug effects Cell Line, Tumor Cell Movement / drug effects Cell Proliferation / drug effects Fermentation HEK293 Cells Humans Lactobacillus plantarum* / metabolism Pancreatic Neoplasms* / drug therapy Pancreatic Neoplasms* / metabolism Pancreatic Neoplasms* / pathology Plant Extracts* / chemistry Plant Extracts* / pharmacology Plant Leaves* / chemistry Stevia* / chemistry |
| IF | 4.556 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | News |
| Total number of mentions | 68 |
| Altmetric score changes over past 6months | 13.0 |
| Resource | |
| Human and Animal Cells | PANC-1(RCB2095) |