| RRC ID |
83699
|
| 著者 |
Weasner BM, Weasner BP, Cook KR, Stinchfield MJ, Kondo S, Saito K, Kumar JP, Newfeld SJ.
|
| タイトル |
A new Drosophila melanogaster research resource: CRISPR-induced mutations for clonal analysis of fourth chromosome genes.
|
| ジャーナル |
G3 (Bethesda)
|
| Abstract |
As part of an ongoing effort to generate comprehensive resources for the experimental analysis of fourth chromosome genes in Drosophila melanogaster, the Fourth Chromosome Resource Project has used CRISPR mutagenesis with single guide RNAs to isolate mutations in 62 of the 80 fourth chromosome, protein-coding genes. These mutations were induced on a fourth chromosome bearing a basal FRT insertion to facilitate experimental approaches involving FLP recombinase-induced mitotic recombination. To permit straightforward comparisons among mutant stocks, most of the mutations were generated on isogenic fourth chromosomes, which were then crossed into a common genetic background. Of the 119 mutations, 84 are frameshift mutations likely to be null alleles, 29 are small, in-frame deletions, and 6 have yet to be characterized molecularly. The mutations were tested for recessive lethal, female-sterile, and visible phenotypes. Stable stocks for most of the mutations have been submitted to repositories in the United States and Japan for public distribution.
|
| 巻・号 |
15(3)
|
| 公開日 |
2025-3-18
|
| DOI |
10.1093/g3journal/jkaf006
|
| PII |
7953301
|
| PMID |
39804955
|
| PMC |
PMC11917476
|
| MeSH |
Animals
CRISPR-Cas Systems*
Chromosomes, Insect / genetics
Clustered Regularly Interspaced Short Palindromic Repeats
Drosophila melanogaster* / genetics
Female
Male
Mutation*
Phenotype
|
| IF |
2.781
|
| リソース情報 |
| ショウジョウバエ |
DGRC#119169
DGRC#119165
DGRC#119160
DGRC#119163
DGRC#119073
DGRC#119193
DGRC#119162
DGRC#119161
DGRC#119170
DGRC#119168
DGRC#119171
DGRC#119074
DGRC#119167
DGRC#119166
DGRC#119192
DGRC#119191
DGRC#119189
DGRC#119190
DGRC#119164
DGRC#119072 |
