| RRC ID |
83776
|
| Author |
Tignard P, Pottin K, Geeverding A, Doulazmi M, Cabrera M, Fouquet C, Liffran M, Fouchard J, Rosello M, Albadri S, Del Bene F, Trembleau A, Breau MA.
|
| Title |
Basement membranes are crucial for proper olfactory placode shape, position and boundary with the brain, and for olfactory axon development.
|
| Journal |
Elife
|
| Abstract |
Despite recent progress, the complex roles played by the extracellular matrix in development and disease are still far from being fully understood. Here, we took advantage of the zebrafish sly mutation which affects Laminin γ1, a major component of basement membranes, to explore its role in the development of the olfactory system. Following a detailed characterisation of Laminin distribution in the developing olfactory circuit, we analysed basement membrane integrity, olfactory placode and brain morphogenesis, and olfactory axon development in sly mutants, using a combination of immunochemistry, electron microscopy and quantitative live imaging of cell movements and axon behaviours. Our results point to an original and dual contribution of Laminin γ1-dependent basement membranes in organising the border between the olfactory placode and the adjacent brain: they maintain placode shape and position in the face of major brain morphogenetic movements, they establish a robust physical barrier between the two tissues while at the same time allowing the local entry of the sensory axons into the brain and their navigation towards the olfactory bulb. This work thus identifies key roles of Laminin γ1-dependent basement membranes in neuronal tissue morphogenesis and axon development in vivo.
|
| Volume |
12
|
| Published |
2024-12-23
|
| DOI |
10.7554/eLife.92004
|
| PII |
92004
|
| PMID |
39713923
|
| PMC |
PMC11666240
|
| MeSH |
Animals
Axons* / metabolism
Axons* / physiology
Basement Membrane* / metabolism
Brain / embryology
Brain / metabolism
Ectodermal Placodes
Laminin / metabolism
Morphogenesis
Mutation
Olfactory Bulb / embryology
Olfactory Bulb / metabolism
Olfactory Mucosa / embryology
Olfactory Mucosa / metabolism
Zebrafish* / embryology
Zebrafish Proteins / genetics
Zebrafish Proteins / metabolism
|
| IF |
7.08
|
| Resource |
| Zebrafish |
Tg(OMP2k :gap-YFP)rw032 |
