RRC ID 839
Author Nishimura R, Hayashi M, Wu GJ, Kouchi H, Imaizumi-Anraku H, Murakami Y, Kawasaki S, Akao S, Ohmori M, Nagasawa M, Harada K, Kawaguchi M.
Title HAR1 mediates systemic regulation of symbiotic organ development.
Journal Nature
Abstract Symbiotic root nodules are beneficial to leguminous host plants; however, excessive nodulation damages the host because it interferes with the distribution of nutrients in the plant. To keep a steady balance, the nodulation programme is regulated systemically in leguminous hosts. Leguminous mutants that have lost this ability display a hypernodulating phenotype. Through the use of reciprocal and self-grafting studies using Lotus japonicus hypernodulating mutants, har1 (also known as sym78), we show that the shoot genotype is responsible for the negative regulation of nodule development. A map-based cloning strategy revealed that HAR1 encodes a protein with a relative molecular mass of 108,000, which contains 21 leucine-rich repeats, a single transmembrane domain and serine/threonine kinase domains. The har1 mutant phenotype was rescued by transfection of the HAR1 gene. In a comparison of Arabidopsis receptor-like kinases, HAR1 showed the highest level of similarity with CLAVATA1 (CLV1). CLV1 negatively regulates formation of the shoot and floral meristems through cell-cell communication involving the CLV3 peptide. Identification of hypernodulation genes thus indicates that genes in leguminous plants bearing a close resemblance to CLV1 regulate nodule development systemically, by means of organ-organ communication.
Volume 420(6914)
Pages 426-9
Published 2002-11-28
DOI 10.1038/nature01231
PII nature01231
PMID 12442172
MeSH Arabidopsis Proteins / chemistry Arabidopsis Proteins / genetics Chromosome Mapping Cloning, Molecular DNA, Plant / analysis DNA, Plant / genetics Genes, Plant / genetics Genetic Complementation Test Lotus / enzymology* Lotus / genetics Lotus / growth & development* Lotus / metabolism Molecular Sequence Data Mutation / genetics Nitrogen Fixation* Plant Roots / enzymology Plant Roots / genetics Plant Roots / growth & development Plant Roots / metabolism Plant Shoots / enzymology Plant Shoots / genetics Plant Shoots / metabolism Protein-Serine-Threonine Kinases / chemistry Protein-Serine-Threonine Kinases / genetics Protein-Serine-Threonine Kinases / metabolism* RNA, Plant / analysis RNA, Plant / genetics Receptor Protein-Tyrosine Kinases / chemistry Receptor Protein-Tyrosine Kinases / genetics Signal Transduction Soybeans / chemistry Soybeans / genetics Symbiosis* Transplants
IF 41.577
Times Cited 294
Lotus / Glycine Gifu B-129 Miyakojima MG-20