論文 - 詳細
| RRC ID | 84074 |
|---|---|
| 著者 | Sandhu A, Lyu X, Wan X, Meng X, Tang NH, Gonzalez G, Syed IN, Chen L, Jin Y, Chisholm AD. |
| タイトル | The microtubule regulator EFA-6 forms cortical foci dependent on its intrinsically disordered region and interactions with tubulins. |
| ジャーナル | Cell Rep |
| Abstract |
The EFA6 protein family, originally identified as Sec7 guanine nucleotide exchange factors, has also been found to regulate cortical microtubule (MT) dynamics. Here, we find that in the mature C. elegans epidermal epithelium, EFA-6 forms punctate foci in specific regions of the apical cortex, dependent on its intrinsically disordered region (IDR). The EFA-6 IDR can form biomolecular condensates in vitro. In genetic screens for mutants with altered GFP::EFA-6 localization, we identified a gain-of-function (gf) mutation in α-tubulin tba-1 that induces ectopic EFA-6 foci in multiple cell types. Lethality of tba-1(gf) is partially suppressed by loss of function in efa-6. The ability of TBA-1(gf) to trigger ectopic EFA-6 foci requires β-tubulin TBB-2 and the chaperon EVL-20/Arl2. tba-1(gf)-induced EFA-6 foci display slower turnover, contain the MT-associated protein TAC-1/TACC, and require the EFA-6 MT elimination domain (MTED). Our results reveal functionally important crosstalk between cellular tubulins and cortical MT regulators in vivo. |
| 巻・号 | 43(10) |
| ページ | 114776 |
| 公開日 | 2024-10-22 |
| DOI | 10.1016/j.celrep.2024.114776 |
| PII | S2211-1247(24)01127-6 |
| PMID | 39305484 |
| PMC | PMC11972086 |
| MeSH | Animals Caenorhabditis elegans* / metabolism Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism Guanine Nucleotide Exchange Factors* / chemistry Guanine Nucleotide Exchange Factors* / genetics Guanine Nucleotide Exchange Factors* / metabolism Intrinsically Disordered Proteins / chemistry Intrinsically Disordered Proteins / metabolism Microtubules* / metabolism Mutation Protein Binding Tubulin* / metabolism |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm3124 tmC27 |