| RRC ID |
84078
|
| Author |
Castiglioni VG, Olmo-Uceda MJ, Villena-Giménez A, Muñoz-Sánchez JC, Legarda EG, Elena SF.
|
| Title |
Story of an infection: Viral dynamics and host responses in the Caenorhabditis elegans-Orsay virus pathosystem.
|
| Journal |
Sci Adv
|
| Abstract |
Orsay virus (OrV) is the only known natural virus affecting Caenorhabditis elegans, with minimal impact on the animal's fitness due to its robust innate immune response. This study aimed to understand the interactions between C. elegans and OrV by tracking the infection's progression during larval development. Four distinct stages of infection were identified on the basis of viral load, with a peak in capsid-encoding RNA2 coinciding with the first signs of viral egression. Transcriptomic analysis revealed temporal changes in gene expression and functions induced by the infection. A specific set of up-regulated genes remained active throughout the infection, and genes correlated and anticorrelated with virus accumulation were identified. Responses to OrV mirrored reactions to other biotic stressors, distinguishing between virus-specific responses and broader immune responses. Moreover, mutants of early response genes and defense-related processes showed altered viral load progression, uncovering additional players in the antiviral defense response.
|
| Volume |
10(39)
|
| Pages |
eadn5945
|
| Published |
2024-9-27
|
| DOI |
10.1126/sciadv.adn5945
|
| PMID |
39331715
|
| PMC |
PMC11430451
|
| MeSH |
Animals
Caenorhabditis elegans* / genetics
Caenorhabditis elegans* / immunology
Caenorhabditis elegans* / virology
Gene Expression Profiling
Gene Expression Regulation
Host-Pathogen Interactions* / genetics
Host-Pathogen Interactions* / immunology
Immunity, Innate
Nodaviridae / physiology
Transcriptome
Viral Load*
|
| Resource |
| C.elegans |
tm6609
tm10539
tm1637
tm2319
tm894
tm2398 |
