論文 - 詳細
| RRC ID | 84201 |
|---|---|
| 著者 | Liu S, Wei J, Zhong L, Hai S, Song S, Xie C, Huang Z, Cheng Z, Zhang J, Du A, Zhang P, Yan Y, Shi A. |
| タイトル | RAB-10 cooperates with EHBP-1 to capture vesicular carriers during post-Golgi exocytic trafficking. |
| ジャーナル | J Cell Biol |
| Abstract |
Post-Golgi exocytic trafficking, fundamental for secretion and cell surface component integration, remains incompletely understood at the molecular level. Here, we investigated this process using Caenorhabditis elegans and mammalian cell models, revealing a novel exocytic carrier capturing mechanism involving the small GTPase RAB-10/Rab10 and its effector EHBP-1/EHBP1. EHBP-1, localized in recycling endosomes, selectively captures RAB-10-positive lipoprotein exocytic carriers through its interaction with active RAB-10, thereby promoting the delivery of exocytic cargo to recycling endosomes. A detailed mechanistic examination demonstrated the synergy between EHBP-1's RAB-10-binding coiled-coil domain and its PI(4,5)P2-binding C2 domain in the capturing process. Of note, we identified LST-6/DENND5 as a specialized guanine nucleotide exchange factor (GEF) for RAB-10 in this particular pathway, distinct from the GEF involved in basolateral recycling. Following the RAB-10-EHBP-1-mediated capture, the exocyst complex carries out its function. Taken together, this study suggests a potential tethering mechanism for basolateral post-Golgi exocytic carriers, highlighting the coordination among membrane compartments in regulating this trafficking route. |
| 巻・号 | 224(4) |
| 公開日 | 2025-4-7 |
| DOI | 10.1083/jcb.202410003 |
| PII | 277283 |
| PMID | 39982707 |
| PMC | PMC11844438 |
| MeSH | Animals Caenorhabditis elegans* / genetics Caenorhabditis elegans* / metabolism Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism Endosomes / metabolism Exocytosis* Golgi Apparatus* / genetics Golgi Apparatus* / metabolism Guanine Nucleotide Exchange Factors / genetics Guanine Nucleotide Exchange Factors / metabolism HeLa Cells Humans Protein Binding Protein Transport Vesicular Transport Proteins* / genetics Vesicular Transport Proteins* / metabolism rab GTP-Binding Proteins* / genetics rab GTP-Binding Proteins* / metabolism |
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 12 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1443 |