論文 - 詳細
| RRC ID | 84740 |
|---|---|
| 著者 | Li S, Li C, Pei J, Liu R, Fang J, Wei Y, He Y, Li S, Feng Q, Zhang C, Guo T, Jiang Y, Hu Y, Jiang Z, Shi L, Dong Y. |
| タイトル | Tepidibacillus marianensis sp. nov., a novel heterotrophic iron-reducing bacterium isolated from Mariana Trench sediment. |
| ジャーナル | Int J Syst Evol Microbiol |
| Abstract |
A novel chemoheterotrophic iron-reducing micro-organism, designated as strain LSZ-M11000T, was isolated from sediment of the Marianas Trench. Phylogenetic analysis based on the 16S rRNA gene revealed that strain LSZ-M11000T belonged to genus Tepidibacillus, with 97 % identity to that of Tepidibacillus fermentans STGHT, a mesophilic bacterium isolated from the Severo-Stavropolskoye underground gas storage facility in Russia. The polar lipid profile of strain LSZ-M11000T consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, as well as other unidentified phospholipids and lipids. The major fatty acids were C16 : 0 (28.4 %), C18 : 0 (15.8 %), iso-C15 : 0 (12.9 %), and anteiso-C15 : 0 (12.0 %). Strain LSZ-M11000T had no menaquinone. Genome sequencing revealed that the genome size of strain LSZ-M11000T was 2.97 Mb and the DNA G+C content was 37.9 mol%. The average nucleotide identity values between strain LSZ-M11000T and its close phylogenetic relatives, Tepidibacillus fermentans STGHT and Tepidibacillus decaturensis Z9T, were 76.4 and 72.6 %, respectively. The corresponding DNA-DNA hybridization estimates were 20.9 and 23.4 %, respectively. Cells of strain LSZ-M11000T were rod-shaped (1.0-1.5×0.3-0.5 µm). Using pyruvate as an electron donor, it was capable of reducing KMnO4, MnO2, As(V), NaNO3, NaNO2, Na2SO4, Na2S2O3, and K2Cr2O7. Based on phenotypic, genotypic, and phylogenetic evidence, strain LSZ-M11000T is proposed to be a novel strain of the genus Tepidibacillus, for which the name Tepdibacillus marianensis is proposed. The type strain is LSZ-M11000T (=CCAM 1008T=JCM 39431T). |
| 巻・号 | 74(6) |
| 公開日 | 2024-6-1 |
| DOI | 10.1099/ijsem.0.006438 |
| PMID | 38913036 |
| MeSH | Bacillaceae / classification Bacillaceae / genetics Bacillaceae / isolation & purification Bacterial Typing Techniques* Base Composition* DNA, Bacterial* / genetics Fatty Acids* Geologic Sediments* / microbiology Heterotrophic Processes Iron* / metabolism Nucleic Acid Hybridization Oxidation-Reduction Phospholipids* Phylogeny* RNA, Ribosomal, 16S* / genetics Russia Sequence Analysis, DNA* Whole Genome Sequencing |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Blogs |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM39431 |