RRC ID 84779
Author Paek J, Bai L, Shin Y, Kim H, Kim SH, Shin JH, Kook JK, Chang YH.
Title Description of Olsenella absiana sp. nov., an anaerobic bacterium isolated from pig faeces.
Journal Int J Syst Evol Microbiol
Abstract A short-rod-shaped, obligate anaerobic, Gram-positive bacterium isolated from the faeces of a pig was designated as the strain YH-ols2223T. Analysis of the 16S rRNA gene sequence revealed that the isolate was most closely related to Olsenella urininfantis KCTC 25755T with 97.7% similarity. The multi-locus sequence tree revealed that the isolate formed a sub-cluster within the genus Olsenella. The average nucleotide identity and the digital DNA-DNA hybridization values for the isolate and the most closely related strains were 74.9-76.6% and 20.8-22.5%, respectively. The end products of the fermentation were lactic acid and acetic acid. The main fatty acids identified were C18 : 0 DMA, C18 : 1 ω9c DMA and C18 : 1 ω9c. The cell wall contained the meso-diaminopimelic acid-based peptidoglycan. Given the chemotaxonomic, phenotypic and phylogenetic properties, YH-ols2223T (= KCTC 25800T = NBRC 116680T) represents a novel taxon. The name Olsenella absiana sp. nov. was proposed.
Volume 74(11)
Published 2024-11-1
DOI 10.1099/ijsem.0.006579
PMID 39531284
MeSH Acetic Acid / metabolism Animals Bacteria, Anaerobic / classification Bacteria, Anaerobic / genetics Bacteria, Anaerobic / isolation & purification Bacterial Typing Techniques* DNA, Bacterial* / genetics Diaminopimelic Acid / analysis Fatty Acids* / analysis Feces* / microbiology Fermentation Lactic Acid / metabolism Multilocus Sequence Typing Nucleic Acid Hybridization* Peptidoglycan* Phylogeny* RNA, Ribosomal, 16S* / genetics Sequence Analysis, DNA* Swine
Resource
General Microbes JCM34369 JCM14553